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| Lima,Juliana O.; Pereira,Jorge F.; Rincones,Johana; Barau,Joan G.; Araújo,Elza F.; Pereira,Gonçalo A.G.; Queiroz,Marisa V.. |
| This report describes the cloning, sequence and expression analysis of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene of Moniliophthora perniciosa, the most important pathogen of cocoa in Brazil. Southern blot analysis revealed the presence of a single copy of the GAPDH gene in the M. perniciosa genome (MpGAPDH). The complete MpGAPDH coding sequence contained 1,461 bp with eight introns that were conserved in the GAPDH genes of other basidiomycete species. The cis-elements in the promoter region of the MpGAPDH gene were similar to those of other basidiomycetes. Likewise, the MpGAPDH gene encoded a putative 339 amino acid protein that shared significant sequence similarity with other GAPDH proteins in fungi, plants, and metazoans. Phylogenetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glyceraldehyde-3-phosphate dehydrogenase gene; Moniliophthora perniciosa; Witches' broom. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200024 |
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| Gomes,Eliane A.; Kasuya,Maria Catarina M.; Barros,Everaldo G. de; Borges,Arnaldo C.; Araújo,Elza F.. |
| Inter- and intraspecific variation among 26 isolates of ectomycorrhizal fungi belonging to 8 genera and 19 species were evaluated by analysis of the internal transcribed sequence (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with different restriction enzymes. Amplification products, which ranged between 560 and 750 base pairs (bp), were obtained for all the isolates analyzed. The degree of polymorphism observed did not allow proper identification of most of the isolates. Cleavage of amplified fragments with the restriction enzymes Alu I, Hae III, Hinf I, and Hpa II revealed extensive polymorphism. All eight genera... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA fingerprinting; Ectomycorrhizal fungi; Internal transcribed spacer (ITS); PCR-RFLP; Ribosomal DNA (rDNA). |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400018 |
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| Pereira,Jorge F.; Ignacchiti,Mariana D.C.; Araújo,Elza F.; Brommonschenkel,Sérgio H.; Cascardo,Júlio C.M.; Pereira,Gonçalo A. G.; Queiroz,Marisa V.. |
| Reverse transcriptase (RT) sequence analysis is an important technique used to detect the presence of transposable elements in a genome. Putative RT sequences were analyzed in the genome of the pathogenic fungus C. perniciosa, the causal agent of witches' broom disease of cocoa. A 394 bp fragment was amplified from genomic DNA of different isolates of C. perniciosa belonging to C-, L-, and S-biotypes and collected from various geographical areas. The cleavage of PCR products with restriction enzymes and the sequencing of various RT fragments indicated the presence of several sequences showing transition events (G:C to A:T). Southern blot analysis revealed high copy numbers of RT signals, forming different patterns among C-, S-, and L-biotype isolates.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic variability; Transposable elements; Witches' broom; Theobroma cacao. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000500001 |
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| Zanotti,Michele G. S.; Santos,Jildete K.; Reis,Kledna C. P.; Araújo,Elza F.; Dhingra,Onkar Dev; Queiroz,Marisa V.. |
| A variabilidade genética de 20 isolados de Fusarium oxysporum, nove não-patogênicos e 11 patogênicos ao feijoeiro (Phaseouls vulgaris), foi determinada com base na distribuição do elemento transponível impala. A presença de impala das subfamílias D e E foi determinada por experimentos de PCR, empregando oligonucleotídeos específicos para cada subfamília. Foi observada a presença de representantes das duas subfamílias na maioria dos isolados, sugerindo, portanto, que impala é um antigo componente do genoma de F. oxysporum f. sp. phaseoli. A hibridização do DNA total de cada isolado, clivado com a enzima EcoRI, com um fragmento do elemento impala da subfamíla E, mostrou uma variação nos padrões de bandas dos isolados não-patogênicos, indicando a possível... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transposon; Murcha de feijoeiro; PCR; Variabilidade genética; Patogenicidade. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000300005 |
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