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| Lima,Francisco Esmaile de Sales; Batista,Helena Beatriz Ruthner; Campos,Fabrício Souza; Kunert Filho,Hiran Castagnino; Cibulski,Samuel; Finoketti,Fernando; Arantes,Thalita; Spilki,Fernando; Roehe,Paulo Michel; Rijsewijk,Franciscus Antonius Maria; Franco,Ana Cláudia. |
| This study aimed to analyze the effect of the expression of Parainfluenza virus 5 (PIV5) V protein in bovine cells on the replication of Bovine herpesvirus 5 (BoHV-5). Growth properties of BoHV-5 were evaluated in parental and PIV5 transfected cells. In one-step growth experiments, the BoHV-5 reached higher titers at earlier time points in the transfected cells when compared to the parental cells. The mean plaque size produced by the BoHV-5 in transfected cells was larger than the parental cells. This indicated that the expression of the PIV5 V gene facilitated the release and cell-to-cell spread of BoHV-5 in bovine cells. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BoHV-5; V mRNA; Transfection. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100007 |
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