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Characterization of two novel sialyl N-acetyllactosaminyl nucleotides separated from ovine colostrum OAK
Sasaki, Masashi; Nakamura, Tadashi; Hirayama, Kentaro; Fukuda, Kenji; Saito, Tadao; Urashima, Tadasu; Asakuma, Sadaki.
The milk/colostrum of some mammalian species is known to contain sugar nucleotides including uridine diphosphate (UDP) oligosaccharides in addition to lactose and milk oligosaccharides, but the detailed structures of these UDP oligosaccharides have not so far been clarified. In this study we isolated two UDP-sialyl N-acetyllactosamines from ovine colostrum and characterized them using H-1-NMR and MALDITOFMS spectroscopies. Their structures were found to be Neu5Gc(alpha 2 - 3) Gal (beta 1 - 4)GlcNAc alpha 1-UDP and Neu5Gc(alpha 2-6) Gal(beta 1-4)GlcNAc alpha 1-UDP.
Palavras-chave: UDP sialyl N-acetyllactosamine; UDP oligosaccharide; Milk sugar nucleotide; Ovine colostrum; Milk oligosaccharide.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4527
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Lactobacillus fermentum TDS030603株の生産する中性多糖の構造研究 OAK
Leo, Fiame; Hashida, Shukichi; Kumagai, Daiju; Uchida, Kenji; Motoshima, Hidemasa; Arai, Ikichi; Asakuma, Sadaki; Fukuda, Kenji; Urashima, Tadasu; 福田, 健二; 浦島, 匡.
A neutral exopolysaccharide produced in large amounts by Lactobacillus fermentum TDS030603 was purified from culture supernatants and its structure determined by monosaccharide analysis, methylation analysis and 1H-NMR spectroscopy. Optimum conditions for the production of this exopolysaccharide by the above bacterial strain, when cultured using cheese whey medium or MRS broth, were also investigated. The exopolysaccharide was found to be composed of D-galactose and D-glucose in the molar ratio of 1:2.5 and to consist of one major as well as one minor saccharide, both of which contained a tetrasaccharide repeating unit consisting of two α and two β anomers. The major saccharide contained non-reducing glucopyranose, O-3 substituted glucopyranose, O-6...
Palavras-chave: Lactic acid bacteria; Exopolysaccharide; Lactobacillus fermentum TDS030603.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4183
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Characterization of oligosaccharides in milk of a mink, Mustela vison OAK
Urashima, Tadasu; Nakamura, Tadashi; Ikeda, Akiko; Asakuma, Sadaki; Arai, Ikichi; Saito, Tadao; Oftedal, Olav T.; 浦島, 匡; 中村, 正.
Carbohydrates were extracted from a sample of milk from a mink, Mustela vison (Family Mustelidae). Free neutral and acidic oligosaccharides were isolated from the carbohydrate fraction and their chemical structures were compared with those of white-nosed coati (Nasua narica, Procyonidae) and harbour seal (Phoca vitulina, Phocidae) that we had studied previously. The ratio of free lactose to milk oligosaccharides was similar to that in milk of the white-nosed coati; in both species, this ratio was much lower than that in the milk of most eutherians. The neutral oligosaccharides of mink milk had alpha(1-3)-linked Gal or alpha(1-2)-linked Fuc residues at their non-reducing ends, as in the neutral oligosaccharides of white-nosed coati milk. Some of the neutral...
Palavras-chave: Mink; Mustela vison; Mustelidae; Milk oligosaccharides; Neutral oligosaccharides; Sialyl oligosaccharides.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/729
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Chemical characterization of the oligosaccharides in Bryde's whale (Balaenoptera edeni) and Sei whale (Balaenoptera borealis lesson) milk OAK
Urashima, Tadasu; Kobayashi, Mami; Asakuma, Sadaki; Uemura, Yusuke; Arai, Ikichi; Fukuda, Kenji; Saito, Tadao; Mogoe, Toshihiro; Ishikawa, Hajime; Fukui, Yutaka.
Samples of milk from a Bryde's whale and a Sei whale contained 2.7 g/100 mL and 1.7 g/100 mL of hexose, respectively. Both contained lactose as the dominant saccharide along with small amounts of Neu5Ac(alpha 2-3)Gal(beta 1-4)Glc(3'-N-acetylneuraminyllactose), Neu5Ac(alpha 2-6)Gal(beta 1-4) Glc(6-N- acetylneuraminyllactose) and Neu5Ac(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-3)Gal(beta 1-4)Glc (LST c). The dominance of lactose in the carbohydrate of these milks is similar to that of Minke whale milk and bottlenose dolphin colostrum, but the oligosaccharide patterns are different from those of these two species, illustrating the heterogeneity of milk oligosaccharides among the Cetacea. (c) 2006 Elsevier Inc. All rights reserved.
Palavras-chave: Bryde's whale; Sei whale; Milk oligosaccharides; Balaenoptera edeni; Balaenoptera borealis lesson; Cetacea.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1659
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