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Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women BJID
de-Paris,Fernanda; Machado,Alice Beatriz Mombach Pinheiro; Gheno,Tailise Conte; Ascoli,Bruna Maria; Oliveira,Kátia Ruschel Pilger de; Barth,Afonso Luis.
Streptococcus agalactiae or group B Streptococcus (GBS) is one of the most important causal agents of serious neonatal infections. Numerous assays have been evaluated for GBS screening in order to validate a fast and efficient method. The aim of this study was to compare the culture technique (established as the gold standard) with the molecular method of polymerase chain reaction (PCR) with specific primers (atr gene). Two hundred and sixty-three samples were analyzed. Vaginal samples were collected, according to the Centers for Disease Control and Prevention (CDC) recommendations, from women over 35 weeks of pregnancy at Hospital de Clínicas de Porto Alegre (HCPA). Two different extraction methods were tested in all samples collected. PCR technique...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptococcus agalactiae; Polymerase chain reaction; Culture; Pregnancy.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400004
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