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| Asinari,F; Cafrune,E.E; Guzmán,F.A; Conci,L.R; Conci,V.C. |
| The vegetative propagation of strawberries favors transmission of systemic pathogens, such as viruses, which are one of the main yield-limiting factors for this crop. More than 20 viruses have been described as infecting this species; one of the most frequent is the Strawberry mottle virus (SMoV), which is responsible for significant economic losses. SMoV is usually detected by reverse transcription polymerase chain reaction (RT-PCR), given that serum is not available for serological tests. In this study, a non-radioactive molecular probe was developed for SMoV detection. The cDNA was synthesized by RT-PCR using specific primers designed from the 3’UTR region of the viral genome. The cloned cDNA segment was labeled and used as a probe. Six RNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fragaria x ananassa; Molecular techniques; Screening viruses; Virus diagnosis. |
| Ano: 2016 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-298X2016000100004 |
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