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Alteração dos metabólitos secundários em plantas de Hypericum perforatum L. (Hypericaceae) submetidas à secagem e ao congelamento Acta Botanica
Diniz,Ana Carolina Boeno; Astarita,Leandro Vieira; Santarém,Eliane Romanato.
Nos últimos anos, o interesse por Hypericum perforatum tem aumentado devido à sua ação antiviral, antidepressiva e moduladora de apoptose em células neoplásicas. O preparo do material vegetal, após ser colhido, envolve freqüentemente a dessecação ou o congelamento e posterior armazenamento, processos que podem alterar o perfil dos metabólitos secundários. Neste sentido, este trabalho avaliou o efeito da secagem, do congelamento e da estocagem sob baixas temperaturas na quantidade de flavonóides e de hipericina nas partes vegetativas de plantas de hipérico. Ramos de hipérico foram submetidos à secagem a 25, 30, 50 e 70 °C, congelados em nitrogênio líquido ou congelados e armazenados a -20 °C por 10, 20 e 30 dias. A quantificação dos flavonóides e de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flavonóides; Hipericina; Metabolismo secundário; Plantas medicinais; Produtos naturais.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062007000200017
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Plant regeneration through somatic embryogenesis of yacón [smallanthus sonchifolius (poepp. and endl.) H. Robinson] BABT
Corrêa,Cynthia Manyra; Oliveira,Graciele Nicolodi de; Astarita,Leandro Vieira; Santarém,Eliane Romanato.
Smallanthus sonchifolius has tuberous roots containing large amounts of fructo-oligosaccharides and its medicinal use has increased due to the hypoglycemic properties reported for this species. An efficient system for propagation via somatic embryogenesis is reported using petiole segments cultivated on MS medium supplemented with combinations of BA, kinetin and 2,4-D, under light and darkness conditions. Embryogenic callus was formed in most of the treatments; however, somatic embryogenesis was promoted by the presence of light. Clusters of somatic embryos appeared on callus surface after 50 days of culture. The highest number of embryos was produced on 0.45 µM BA and 4.5 µM 2,4-D. Embryogenic calli were maintained on MS medium containing 4.5 µM BA and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plant; Tissue culture; Polymnia sonchifolia; Micropropagation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300005
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Nephroprotective and anti-inflammatory effects of aqueous extract of Melissa officinalis L. on acetaminophen-induced and pleurisy-induced lesions in rats BABT
Müzell,Denise Pereira; Lunardelli,Adroaldo; Leite,Carlos Eduardo; Fagundes,Rodrigo Medeiros; Saciura,Vasyl Custódio; Reichel,Carlos Luiz; Oliveira,Jarbas Rodrigues de; Astarita,Leandro Vieira.
This study assessed the bioactive properties of an aqueous extract of M. officinalis for its anti-inflammatory activity and its protection against hepatic and renal lesions induced by acetaminophen (APAP). Animals pre-treated with the crude extract in pleurisy induced by carrageenan showed a reduction in the amounts of exudate, in the numbers of leukocytes and polymorphonuclear cells. Intragastric administration of the extract for seven days prior to the APAP-induced lesion showed no protective effect on the liver. The treatment with the extract induced an increase of serum aspartate aminotransferase, indicating a rise of toxicity. Contrarily, the same treatment reduced the APAP induced lesion in kidney, with respect to ν-glutamyltransferase. The results...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acetaminophen; Melissa officinalis; Pleurisy; Anti-inflammatory; Kidney; Liver.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300006
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Multiple shoot formation in Hypericum perforatum L.and hypericin production Braz. J. Plant Physiol.
Santarém,Eliane Romanato; Astarita,Leandro Vieira.
Hypericum perforatum is a traditional medicinal plant with wound healing and antidepressive properties. Among the secondary compounds of interest is hypericin, a naphtodianthrone that seems to participate in the medicinal effects of this species. The aim of this work was to obtain an efficient micropropagation system of H. perforatum and to compare the hypericin content between in vitro and field-grown plants. Cultures were initiated from nodal segments of mature plants inoculated onto MS medium supplemented with 4.5 muM BA, kinetin, thidiazuron, individually or in combination with 0.05 muM NAA. Organogenic explants were observed on medium with either BA or kinetin alone or in combination of these with NAA. Subculture of organogenic explants onto the...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Medicinal plant; Micropropagation; Organogenesis; St. John's Wort.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000100006
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Long-term maintenance of in vitro cultures affects growth and secondary metabolism of St. John's Wort Ciência Rural
Figueiró,Adriana de Andrade; Correa,Cynthia Manira; Astarita,Leandro Vieira; Santarém,Eliane Romanato.
Hypericum perforatum is a traditional medicinal plant with wound healing and antidepressant properties. Efficiency of micropropagation is often related to the long term maintenance of tissues in culture, which may alter the secondary metabolism of plants. The objective of this study was to evaluate growth and secondary metabolism of in vitro shoots of H. perforatum on short and long term maintenance of cultures (30 and 100 days). The effect of BA and NAA supplementation was evaluated during 30 days of culture. Adventitious shoots were cultivated on MS medium supplemented with 4.4mM BA alone or in combination with 0.05mM NAA for 30 days. A hormone-free medium was used as control. Shoots cultivated for 100 days were maintained in presence of 4.4mM BA....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tissue culture; Hypericum perforatum; Hypericin; Phenolic compounds.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001000010
Registros recuperados: 5
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