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García-Coronado,Heriberto; Troncoso-Rojas,Rosalba; Tiznado-Hernández,Martín Ernesto; de la Cruz Otero,María del Carmen; Díaz-Camacho,Sylvia Páz; Báez-Flores,María Elena. |
Background Isothiocyanates (ITCs) are natural products obtained from plants of the Brassicas family. They represent an environmentally friendly alternative for the control of phytopathogenic fungi. However, as it has been observed with synthetic fungicides, the possibility of inducing ITC-resistant strains is a major concern. It is, therefore, essential to understanding the molecular mechanisms of fungal resistance to ITCs. We analyzed a subtractive library containing 180 clones of an Alternaria alternata strain resistant to 2-propenyl ITC (2-pITC). After their sequencing, 141 expressed sequence tags (ESTs) were identified using the BlastX algorithm. The sequence assembly was carried out using CAP3 software; the functional annotation and metabolic pathways... |
Tipo: Journal article |
Palavras-chave: Blast2GO analysis; Fungal drug tolerance; Isothiocyanates; Natural fungicides. |
Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400011 |
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