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Registros recuperados: 7
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Structural and ultrastructural characterization of zebu (Bosindicus) spermatozoa Biocell
Aquino Luque,Maria Carolina; Báo,Sônia Nair.
The ultrastructure of normal, ejaculated spermatozoa of Bos indicus was studied by means of electron microscopy, being evaluated in two principal parts, the head and the tail. The head is flat, oval or paddle-shaped with a square base, which provides a concave recess for the insertion of the tail. The acrosome tightly covers the anterior two thirds of the nucleus. A distinct unilateral acrosomal bulge was observed along the apical edge of the head. The equatorial region demarcates the acrosome from the post-equatorial region that covers the caudal one third of the nucleus. The classical 9+9+2 fiber pattern which composes the axoneme was observed along three segments of the tail, namely middle, principal and terminal pieces. The axoneme is anteriorly bound...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spermatozoon; Ultrastructure; Bos indicus.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100005
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An ultrastructural study of spermiogenesis in two species of Sitophilus: Coleoptera: Curculionidae Biocell
Name,Khesller Patrícia Olázia; Dos Reis,Giuliano Pagy Felipe; Báo,Sônia Nair.
The spermiogenesis of Sitophilus zeamais and Sitophilus oryzae, the maize and the rice weevil, respectively, was studied by light microscopy and scanning and transmission electron microscopy. Sitophilus spp. is the most widespread and destructive primary pest of stored cereals in the world. The spermiogenesis occurs within cysts. There are approximately 256 germ line cells per cyst. Inside each cysts, all the spermatids are in the same stage of maturation. The ultrastructure of the spermatozoa of S. zeamais and S. oryzae is similar to that described for other beetles. The head is formed by a three-layered acrosome with the perforatorium, the acrosomal vesicle, the extra-acrosomal layer and the nucleus. The flagellum has the typical axoneme formed by a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Curculionidae; Sitophilus; Spermiogenesis; Insect sperm; Ultrastructure.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000200005
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Leaf surfaces of Gomphrena spp. (Amaranthaceae) from Cerrado biome Biocell
Carvalho,Suzane Margaret Fank-de-; Gomes,Misléia Rodrigues de Aguiar; Silva,Pedro Ítalo Tanno; Báo,Sônia Nair.
The leaf structure and micromorphology characterize plant species and reflex its interactions with the environment. Leaf epidermis sculptures aid high transpiration plants on light reflection. The form and distribution of epicuticular wax crystalloids are important to characterize the surface. Aiming to know the micromorphology and the ultrastructure of G. arborescens, G. pohlii and G. virgata, leaves of these Cerrado native species were collected in Brasília, Distrito Federal, Brazil, at the Olympic Center of the Universidade de Brasília and at Reserva Ecológica do Roncador. Leaves of G. globosa, an Indian native species, were also studied for comparison. Leaves were fractionated, fixed and treated for observation under optical and scanning electron...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anatomy; Epicuticular wax; Platelets; Trichomes; Stomata.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100004
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Anatomia foliar, morfologia e aspectos ecológicos das espécies da família Amaranthaceae da Reserva Particular do Patrimônio Natural Cara Preta, em Alto Paraíso, GO, Brasil Biota Neotropica
Fank-de-Carvalho,Suzane Margaret; Marchioretto,Maria Salete; Báo,Sônia Nair.
A família Amaranthaceae é formada por cerca de 2.360 espécies, 145 delas encontradas no Brasil. Cerca de 94 espécies da família subsistem em diversas fitofisionomias do Bioma Cerrado e 27 espécies aparecem em listas regionais de espécies ameaçadas de extinção. O objetivo deste trabalho foi inventariar e estudar a anatomia foliar e a morfologia de espécies da família Amaranthaceae de uma Unidade de Conservação de Alto Paraíso, GO, relacionando-as ao metabolismo fotossintético. Foram localizadas uma espécie de hábito subarbustivo (Pfaffia townsendii) e cinco espécies herbáceas (Froelichiella grisea, Gomphrena hermogenesii, G. lanigera, G. prostrata e P. gnaphalioides), a maioria demonstrando comportamento pirofítico e anemocoria, bem como sistemas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; Anatomia kranz; Morfologia; Froelichiella; Gomphrena; Pfaffia.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032010000400011
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Morphological alterations and G0/G1 cell cycle arrest induced by curcumin in human SK-MEL-37 melanoma cells BABT
Carneiro,Marcella Lemos Brettas; Porfírio,Elaine Paulucio; Otake,Andréia Hanada; Chammas,Roger; Báo,Sônia Nair; Guillo,Lidia Andreu.
The aim of this work was to study the effect of curcumin on cell cycle in the human SK-MEL-37 melanoma cell line. In addition, morphological and structural analyses were also performed. Flow cytometric analysis showed a G0/G1 arrest at 5 µM after 24 h exposure and a concentration-dependent increase in the proportion of sub-G0 hypodiploid cells. Typical apoptotic events were also observed by the fluorescence microscopy, transmission and scanning electronic microscopy. Loss of mitochondrial membrane potential was not detected. Results suggested that curcumin could arrest human melanoma cells at G0/G1 phase and induce a mitochondrial-independent apoptotic pathway.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Curcumin; Melanoma cells; Apoptosis; Cell cycle.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200013
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Application of xylanases from Amazon Forest fungal species in bleaching of eucalyptus kraft pulps BABT
Medeiros,Roseli Garcia; Silva Jr.,Francides Gomes da; Báo,Sônia Nair; Hanada,Rogério; Ferreira Filho,Edivaldo Ximenes.
Crude xylanase preparations from Penicillium corylophilum, Aspergillus niger and Trichoderma longibrachiatum were used to treat Eucalyptus kraft pulp, prior to chlorine dioxide and alkaline bleaching sequences. The enzyme pretreatment improved brightness and delignification of non-delignified and oxygen-bleached samples of eucalyptus kraft pulp. Xylanase preparations from T. longibrachiatum and P. corylophilum were more effective to reduce pulp kappa number. A small reduction in viscosity was obtained when the oxygen-bleached pulp was treated with xylanase preparation from A. niger. For all enzyme samples, the best release of chromophoric material from the pulp was at 237 nm. The enzyme preparation from P. corylophilum was responsible for the highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylan; Xylanase; Kraft pulp; Pulp bleaching.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200008
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Isolation and characterization of mesenchymal stem cells derived from bovine Wharton's jelly and their potential for use in cloning by nuclear transfer Ciência Rural
Silva,Carolina Gonzales da; Martins,Carlos Frederico; Cardoso,Tereza Cristina; Cunha,Elisa Ribeiro da; Bessler,Heidi Christina; McManus,Concepta Margaret; Pivato,Ivo; Báo,Sônia Nair.
ABSTRACT: Wharton's jelly is a source of mesenchymal stem cells (MSCs) that had not yet been tested for bovine embryo production by nuclear transfer (NT). Thus, the objective of this study was to isolate, characterize and test MSCs derived from Wharton's jelly for embryo and pregnancy production by NT in cattle. The umbilical cord was collected during calving and cells derived from Wharton's jelly (WJCs) were isolated by explant and cultured in Dulbecco's Modified Eagle Medium. Skin Fibroblasts (FB) were isolated after 6 months of life. Morphological analysis was performed by bright field and scanning electron microscopy (SEM) during cell culture. Phenotypic and genotypic characterization by flow cytometry, immunocytochemistry, RT-PCR and differentiation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cloning; Mesenchymal stem cell; Umbilical cord.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001001830
Registros recuperados: 7
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