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Isolation and characterization of selenate resistant mutants of Acremonium chrysogenum BABT
Vialta,Airton; Catani,Cleide F; B. Junior,Renato; Azevedo,João L.
Mutants unable to convert exogenous sulfate to sulfite were isolated using the toxic analogue selenate. Three of twenty-eight isolated mutants were chromate sensitive. They showed a possible lesion in the gene that codes the ATP sulfurylase. The others were chromate resistant, and probably had a lesion in one or both of the genes that code the sulfate permease. Methionine increased the resistance levels to selenate. In addition, the frequency of spontaneous mutants obtained in a medium containing methionine was higher (between 2.4 x 10-6 and 18.0 x 10-6) than that obtained using a medium without any intentional source of sulfur (between 0.7 x 10-6 and 5.0 x 10-6). The original strain, as well as the mutants, were able to grow in a sulfur-free liquid medium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Selenate resistant mutant; Acremonium chrysogenum; Cephalosporin C.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300016
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