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| BALDASSI,L.; BARBOSA,M. L.; BACH,E. E.; IARIA,S. T.. |
| Clinical samples from 71 bovine from different Brazilian states were processed for the analysis of anaerobe organisms with emphasis on the isolation and characterization of Clostridium spp. From these, eighty-nine Clostridium perfringens strains were recovered: 32 from liver, 19 from intestinal contents, 14 from kidney, 6 from rumen, 5 from nervous system, 4 from bone marrow, 2 from udder tract, blood, spleen and lung, and one from muscle. Four reference Clostridium perfringens types A, B, C, and D were used as controls in this study. All isolates were cultivated in appropriate media, and after centrifugation the supernatant and sediment were separated. From pure supernatant post exopolysaccharide (EPS) extraction, mouse toxigenicity tests were performed,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium perfringens; Toxigenicity; Bovine isolates; ELISA. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100010 |
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| BACH,E. E.; KIMATI,H.. |
| Low molecular weight metabolites produced by Bipolaris bicolor, Bipolaris sorokiniana, and Drechslera tritici-repentis are considered to be toxins that facilitate disease in wheat cultivars. Several such toxins were isolated from these fungi. Electrophoresis demonstrated bands of proteins that reduced shoot inhibition in susceptible plants but not in resistant plants. Chlorophyll content was reduced during the first 10 hours of light in the susceptible plants and after 18 hours in the resistant plants. The enzyme beta-1,3-glucanase increased in the resistant plants after treatment with toxins, but in the susceptible plants this enzyme decreased compared to the control. This suggests that the toxin was a protein and the susceptible plants needed other... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxin; Wheat pathogens; Protein toxin. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301999000200006 |
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