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| REYES,EUGENIO; BARAHONA,SALVADOR; FISCHMAN,OLGA; NIKLITSCHEK,MAURICIO; BAEZA,MARCELO; CIFUENTES,VÍCTOR. |
| In this work 20 clinical and 3 environmental yeast isolates were characterized by classical morphological and physiological methods, as well as molecular methods based on PCR of the ITS1-5.8S rDNA-ITS2 region. The characteristic morphology and biochemical profiles observed in these samples correspond to those described for the Pichia genera, more specifically to P. anomala. The profiles of susceptibility to five antifungal drugs were determined by two broth dilution methods. The results obtained by both methods were comparable and showed that clinical isolates presented more resistance to azoles, amphotericin B, and 5-fluorocytosine, than environmental ones did. By amplification and sequencing of internal transcribed spacers (ITS1 and ITS2) and the... |
| Tipo: Journal article |
Palavras-chave: Pichia anomala; 5.8S rDNA; RAPD; Internal transcribed spacer; Antifungal drugs. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500004 |
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| NIKLITSCHEK,MAURICIO; ALCAÍNO,JENNIFER; BARAHONA,SALVADOR; SEPÚLVEDA,DIONISIA; LOZANO,CARLA; CARMONA,MARISELA; MARCOLETA,ANDRÉS; MARTÍNEZ,CLAUDIO; LODATO,PATRICIA; BAEZA,MARCELO; CIFUENTES,VÍCTOR. |
| The cloning and nucleotide sequence of the genes (idi, crtE, crtYB, crtl and crtS) controlling the astaxanthin biosynthesis pathway of the wild-type ATCC 24230 strain of Xanthophyllomyces dendrorhous in their genomic and cDNA version were obtained. The idi, crtE, crtYB, crtl and crtS genes were cloned, as fragments of 10.9, 11.5, 15.8, 5.9 and 4 kb respectively. The nucleotide sequence data analysis indicates that the idi, crtE, crtYB, crtl and crtS genes have 4, 8,4, 11, and 17 introns and 5, 9, 5, 12 and 18 exons respectively. In addition, a highly efficient site-directed mutagenesis system was developed by transformation by integration, followed by mitotic recombination (the double recombinant method). Heterozygote idi (idi+ / idi-::hph), crtE (crtE+ /... |
| Tipo: Journal article |
Palavras-chave: Astaxanthin biosynthesis; Xanthophyllomyces dendrorhous; Phaffia rhodozyma. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100011 |
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| LODATO,PATRICIA; ALCAÍNO,JENNIFER; BARAHONA,SALVADOR; RETAMALES,PATRICIO; JIMÉNEZ,ANTONIO; CIFUENTES,VÍCTOR. |
| The expression, at the mRNA level, of carotenoid biosynthetic genes from Xanthophyllomyces dendrorhous was studied by RT-PCR. The experimental conditions for the RT-PCR assay were standardized to quantify the relative transcript levels of idi, crtE, crtYB and crtI genes. This work attempted to correlate astaxanthin production with the transcript levels of carotenogenic genes in a wild-type strain (UCD 67-385) and two overproducer deregulated strains (atxS1 and atxS2). At 3 day cultures, the wild-type strain contained higher transcript levels from the crtE and crtYB genes on minimal medium than on rich medium. Similarly, carotenoid production was higher on minimal medium than on rich medium. However, carotenoid production in the atxS1 and atxS2 strains was... |
| Tipo: Journal article |
Palavras-chave: Carotenoid biosynthesis; Overproducer strains; Xanthophyllomyces dendrorhous; Phaffia rhodozyma. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100009 |
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