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Oxidative stress responses of juvenile tambaqui Colossoma macropomum after short-term anesthesia with benzocaine and MS-222 Anais da ABC (AABC)
STRINGHETTA,GIOVANNA R.; BARBAS,LUIS A.L.; MALTEZ,LUCAS C.; SAMPAIO,LUÍS A.; MONSERRAT,JOSÉ M.; GARCIA,LUCIANO O..
ABSTRACT The present study aimed to evaluate the effects of benzocaine and tricaine methanesulfonate on oxidative stress parameters of juvenile tambaqui tissues. Fish (n=80) were anesthetized with benzocaine (100 mg L-1) or tricaine (240 mg L-1) and two control groups were used (non-anesthetized fish and fish exposed to ethanol-only). After anesthetic induction 10 fish/anesthetic were euthanized after 3, 12 and 24 hours post-anesthesia and tissue samplings (gills, liver and brain) were performed. Samples were submitted to analyses of enzyme activity glutathione-S-transferase (GST), cellular lipid peroxidation (TBARS) and total antioxidant capacity (ACAP). ACAP increased in gills of benzocaine treatment after 12 hours. The liver showed a reduction in ACAP...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anesthetics; Fish; GST; Lipid peroxidation; Total antioxidant capacity.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502209
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