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	Provedor de dados Trop. Plant Pathol. (2) Autor BELLATO,CLÁUDIA M. (2) MEINHARDT,LYNDEL W. (2) TSAI,SIU M. (2) WULFF,NELSON A. (2) Palavra-chave Genetic biodiversity (1) Genetic diversity (1) ITS (1) Mata Atlântica (1) Mycelial compatibility group (1) Phaseolus vulgaris (1) Soil-borne fungi (1) Telomere (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2002 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Genetic analyses of Rhizoctonia solani isolates from Phaseolus vulgaris grown in the Atlantic Rainforest Region of São Paulo, Brazil Trop. Plant Pathol. MEINHARDT,LYNDEL W.; WULFF,NELSON A.; BELLATO,CLÁUDIA M.; TSAI,SIU M.. Rhizoctonia solani isolates obtained from common beans (Phaseolus vulgaris) grown in the mountainous Atlantic Rainforest (Mata Atlântica) region of São Paulo, Brazil, were analyzed to determine their genetic diversity using internal transcribed spacer (ITS), microsatellite and telomere sequence-based PCR primers. Restriction digestion of the ITS1/5.8S/ITS2 ribosomal regions yielded unique banding patterns specific for AG4 and its subgroups. The ITS restriction digestion (ITS/RFLP), telomere and microsatellite primers identified five to 11 genotypes within the isolates of R. solani. While all isolates were pathogenic on beans, there was no correlation found between genotypic differences and pathogenicity. The different PCR primers revealed a number of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mata Atlântica; Genetic biodiversity; Soil-borne fungi; ITS; Telomere. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000300004 Telomere and microsatellite primers reveal diversity among Sclerotinia sclerotiorum isolates from Brazil Trop. Plant Pathol. MEINHARDT,LYNDEL W.; WULFF,NELSON A.; BELLATO,CLÁUDIA M.; TSAI,SIU M.. Sclerotinia sclerotiorum, the causal agent of white mold, is a problem of winter bean (Phaseolus vulgaris) production in Brazil under center-pivot irrigation. Isolates of S. sclerotiorum were obtained from a center-pivot-irrigated field near Guaíra-SP, Brazil. Mycelial compatibility group (MCG) studies revealed the presence of only two MCG. PCR/RFLP analysis of the ITS1-5.8S-ITS2 ribosomal subunit regions of these field isolates of S. sclerotiorum failed to show any genetic differences between these two MCGs. DNA amplification with a chromosomal telomere sequence-based primer and one microsatellite primer revealed genetic polymorphisms among isolates within the same MCG. Isolates taken from beans and two other crops from another region of Brazil showed the... Tipo: Info:eu-repo/semantics/other Palavras-chave: Genetic diversity; Mycelial compatibility group; Phaseolus vulgaris. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000200015 Registros recuperados: 2 Primeira ... 1 ... Última

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