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	Provedor de dados Anais da ABC (AABC) (2) Autor BERTANI,GIOVANI R. (2) GIL,LAURA H.V.G. (2) CARVALHO,AMANDA G.O. (1) CORDEIRO,MARLI T. (1) KASSAR,TELISSA C. (1) MAGALHÃES,TEREZA (1) MARQUES,ERNESTO T.A. (1) QUEIROZ,SABRINA R.A. (1) S. JÚNIOR,JOSÉ V.J. (1) SANTOS,JEFFERSON J.S. (1) SILVA,ANDRÉA N.M.R. DA (1) Mais... Palavra-chave Dengue virus (1) Gaussia luciferase (1) Homologous recombination in yeast (1) Infectious clone (1) Molecular cloning (1) Reporter gene (1) Reverse genetics (1) Yellow fever virus (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2017 (1) 2014 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A two-plasmid strategy for engineering a dengue virus type 3 infectious clone from primary Brazilian isolate Anais da ABC (AABC) SANTOS,JEFFERSON J.S.; CORDEIRO,MARLI T.; BERTANI,GIOVANI R.; MARQUES,ERNESTO T.A.; GIL,LAURA H.V.G.. Dengue infections represent one of the most prevalent arthropod-borne diseases worldwide, causing a wide spectrum of clinical outcomes. Engineered infectious clone is an important tool to study Dengue virus (DENV) biology. Functional full-length cDNA clones have been constructed for many positive-strand RNA viruses and have provided valuable tools for studying the molecular mechanisms involved in viral genome replication, virion assembly, virus pathogenesis and vaccine development. We report herein the successful development of an infectious clone from a primary Brazilian isolate of dengue virus 3 (DENV3) of the genotype III. Using a two-plasmid strategy, DENV3 genome was divided in two parts and cloned separately into a yeast-bacteria shuttle vector. All... Tipo: Info:eu-repo/semantics/article Palavras-chave: Reverse genetics; Dengue virus; Molecular cloning; Infectious clone. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401749 Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase Anais da ABC (AABC) KASSAR,TELISSA C.; MAGALHÃES,TEREZA; S. JÚNIOR,JOSÉ V.J.; CARVALHO,AMANDA G.O.; SILVA,ANDRÉA N.M.R. DA; QUEIROZ,SABRINA R.A.; BERTANI,GIOVANI R.; GIL,LAURA H.V.G.. ABSTRACT Yellow fever is an arthropod-borne viral disease that still poses high public health concerns, despite the availability of an effective vaccine. The development of recombinant viruses is of utmost importance for several types of studies, such as those aimed to dissect virus-host interactions and to search for novel antiviral strategies. Moreover, recombinant viruses expressing reporter genes may greatly facilitate these studies. Here, we report the construction of a recombinant yellow fever virus (YFV) expressing Gaussia luciferase (GLuc) (YFV-GLuc). We show, through RT-PCR, sequencing and measurement of GLuc activity, that stability of the heterologous gene was maintained after six passages. Furthermore, a direct association between GLuc... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gaussia luciferase; Homologous recombination in yeast; Reporter gene; Yellow fever virus. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502119 Registros recuperados: 2 Primeira ... 1 ... Última

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