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MOLECULAR EXCLUSION CHROMATOGRAPHY OF CRUDE VENOM AS AN AUXILIARY TOOL TO IDENTIFY HYBRID HONEYBEE POPULATIONS J. Venom. Anim. Toxins
PALMA,M.S.; BROCHETTO-BRAGA,M.R.; CHAUD-NETTO,J.; MALASPINA,O.; OLIVEIRA,M.R..
A comparison among the profiles of molecular exclusion chromatography in Sephadex G 100 column of venoms from Apis mellifera adansonii and Africanized honeybees revealed unique peaks which might be used to identify these populations. The venoms from hybrid populations resulting from the reciprocal mating of Apis mellifera adansonii and Africanized honeybees presented unique peaks, probably resulting from a synergistic effect between the parental genomes. The occurrence of characteristic peaks in venoms of hybrid populations might be used to identify these populations as well as to distinguish them from their parents.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apis mellifera; Venom; Hybrid populations; Molecular exclusion chromatography; Honeybees.
Ano: 1995 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301995000100004
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THE EFFECT OF A POLYDNAVIRUS OF APANTELES GALLERIAE ON THE HEMOLYMPH PROTEINS OF HOST GALLERIA MELLONELA J. Venom. Anim. Toxins
BROCHETTO-BRAGA,M.R.; PALMA,M.S.; RIBEIRO,J.C.; GOBBI,N..
The presence of a polydnavirus in the ovary of the microhymenoptera Apanteles galleriae was detected. This wasp parasites the larvae of the wax moth Galleria mellonella. To study the possible viral effects on the total protein of G.mellonella, hemolymph extracts of the ovary calyxes of parasitized A. galleriae were injected into 6th and 7th instar cells of G.mellonella larvae. Protein profiles on polyacrilamide gel electrophoresis containing sodium dodecyl sulfate (SDS-PAGE) of host cell extracts showed a suppression of several protein bands when infection occurred in the 6th instar. However, in the 7th instar, parasitism caused an amplification in the synthesis of most proteins with some changes in the electrophoretic profile.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microhymenoptera; Wax moth; Parasitism; Polydnavirus; SDS-PAGE; Apanteles galleriae; Galleria mellonela.
Ano: 1995 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301995000200005
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