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Cloning and chloroplast-targeted expression studies of insect-resistant gene with ricin fusion-gene under chloroplast transit peptide in cotton Electron. J. Biotechnol.
Kiani,Sarfraz; Ali,Arfan; Bajwa,Kamran Shehzad; Muzaffar,Adnan; Ashraf,Muhammad Aleem; Samiullah,Tahir Rehman; Shahid,Ahmad Ali; Husnain,Tayyab.
Background: Transgenic plants inhabiting single Bt gene are prone to develop insect resistance and this resistance has been reported in case of some important yield-devastating insect larvae of commercial crops, such as cotton and rice. Therefore, it has become essential to adapt new strategies to overcome the problem of insect resistance and these new strategies should be sophisticated enough to target such resistant larvae in broad spectrum. Among these, plants may be transformed with Bt gene tagged with some fusion-protein gene that possesses lectin-binding capability to boost the binding sites for crystal protein gene within insect mid-gut in order to overcome any chances of insect tolerance against Bt toxin. Enhanced chloroplast-targeted Bt gene...
Tipo: Journal article Palavras-chave: Chloroplast expression; Chloroplast transit peptide; Cotton transformation; Confocal microscopy; Ricin b-chain.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600013
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