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| Aral,Cenk; Akkiprik,Mustafa; Kaya,Handan; Ataizi-Çelikel,Çigdem; Çaglayan,Sinan; Özisik,Gökhan; Baloglu,Hüseyin; Özer,Ayse. |
| Recently, efforts have been focused on mitochondrial DNA changes and their relation to human cancers. Among them, a 4977 bp deletion of mitochondrial DNA, named "common deletion", has been investigated in several types of tumors, with inconsistent results. In this study, we investigated the presence of the common deletion in tissues from 25 breast, 25 colorectal and 50 thyroid tumors and in the adjacent healthy tissues from Turkish patients. Samples from healthy volunteers were also evaluated for comparison. Two PCR-based methods were used for the detection of the common deletion. First, two pairs of primers were used to amplify wild-type and deleted mtDNA. Then, a highly sensitive nested-PCR was performed, to determine low amounts of deleted genomes. By... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mitochondrial DNA; Common deletion; Cancer. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100001 |
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| Güllü,Gökçe; Peker,Irem; Haholu,Aptullah; Eren,Fatih; Küçükodaci,Zafer; Güleç,Bülent; Baloglu,Hüseyin; Erzik,Can; Özer,Ayse; Akkiprik,Mustafa. |
| The functional role of IGFBP5 in breast cancer is complicated. Experimental and bioinformatics studies have shown that IGFBP5 is targeted by miR-140-5p and miR-193b, although this has not yet been proven in clinical samples. The aim of this study was to evaluate the expression of miR-140-5p and miR-193b in breast cancer and adjacent normal tissue and assess its correlation with IGFBP5 and the clinicopathological characteristics of the tumors. IGFBP5 protein expression was analyzed immunohistochemically and IGFBP5, miR-140 and miR-193b mRNA expression levels were analyzed with real-time RT-PCR. Tumor tissue had higher miR-140-5p expression than adjacent normal tissue (p = 0.015). Samples with no immunohistochemical staining for IGFBP5 showed increased... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breast cancer; ER alpha; IGFBP5; Micro RNA; MiR-140; MiR-193b. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100021 |
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