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Registros recuperados: 11
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Growth Characteristics Modeling of Mixed Culture of Bifidobacterium bifidum and Lactobacillus acidophilus using Response Surface Methodology and Artificial Neural Network BABT
Meena,Ganga Sahay; Majumdar,Gautam Chandra; Banerjee,Rintu; Kumar,Nitin; Meena,Pankaj Kumar.
Different culture conditions viz. additional carbon and nitrogen content, inoculum size and age, temperature and pH of the mixed culture of Bifidobacterium bifidum and Lactobacillus acidophilus were optimized using response surface methodology (RSM) and artificial neural network (ANN). Kinetic growth models were fitted for the cultivations using a Fractional Factorial (FF) design experiments for different variables. This novel concept of combining the optimization and modeling presented different optimal conditions for the mixture of B. bifidum and L. acidophilus growth from their one variable at-a-time (OVAT) optimization study. Through these statistical tools, the product yield (cell mass) of the mixture of B. bifidum and L. acidophilus was increased....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Response surface methodology (RSM); Artificial neural network (ANN); Genetic algorithms (GA); Fractional factorial design (FFD); Bifidobacterium bifidum; Lactobacillus acidophilus.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600962
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Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313 BABT
Palit,Soumen; Banerjee,Rintu.
The extraction of alpha-amylase in the solid state fermentation of wheat bran by Bacillus circulans GRS313 was optimized. Among various solvents tested, maximum extraction was achieved when 2.5% (v/v) glycerol was added. The optimum conditions for extraction were 2.5 hrs soaking time at 30° C under recirculation and agitated condition, which yielded 590 U/g bran of enzyme activity. Whereas under stationary soaking condition the activity of the enzyme was 439.58U/g bran. With repeated wash under the above optimum conditions showed that 600 U/g and 184.2 U/g of enzyme extracted in the 1st and 2nd washes respectively and only 36.84 U/g was recovered in the 3rd wash
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus circulans; Solid state fermentation; Alpha- amylase; Extraction.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100015
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Enhanced lipase recovery through RSM integrated differential evolutionary approach from the fermented biomass BABT
Garlapati,Vijay Kumar; Vundavilli,Pandu Ranga; Banerjee,Rintu.
The aim of this work was to apply a modeling integrated optimisation approach for a complex, highly nonlinear system for an extracellular lipase extraction process. The model was developed using mutation, crossover and selection variables of Differential Evolution (DE) based on central composite design of Response Surface Methodology. The experimentally validated model was optimized by DE, a robust evolutionary optimization tool. A maximum lipase activity of 134.13 U/gds (more than 36.28 U/gds compared to one variable at a time approach) was observed with the DE-stated optimum values of 25.01% dimethyl sulfoxide concentration, 40 mM buffer, 128.52 min soaking time and 35ºC with the DE control parameters, namely number of population, generations, crossover...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Modeling; Optimization; Lipase extraction; Response surface methodology; Differential evolution; Solid state fermentation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500001
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Multivariable parameter optimization for the endoglucanase production by Trichoderma reesei Rut C30 from Ocimum gratissimum seed BABT
Das,Mithu; Banerjee,Rintu; Bal,Satish.
The aim of this study was to evaluate the interaction effects of the physico-chemical parameters on the endoglucanase (CMCase) production by Trichoderma reesei Rut C30 on a cellulosic agro-residue by the solid-state fermentation (SSF) and to determine their optimum values by the EVOP factorial design technique. The best combination of physical parameters for the maximum production of the endoglucanase (CMCase) was 28ºC temperature, 79% relative humidity and 4.8 pH of the medium. The best combination of the chemical parameters was (mg/L) nicotinic acid 15, naphthalene acetic acid 7, ferric chloride 5 and Tween-80 6. With the application of this technique, the yield of the CMCase increased by ~ 2.3 fold.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme; Endoglucanase; Carboxymethylcellulase (CMCase); EVOP factorial design technique.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100005
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Immobilization of alpha-amylase produced by Bacillus circulans GRS 313 BABT
Dey,Gargi; Bhupinder,Singh; Banerjee,Rintu.
A maltooligosaccharide-forming amylase from B circulans GRS 313 was immobilized by entrapment in calcium alginate beads. The immobilized activity was affected by the size of the bead and bead size of 2mm was found to be most effective for hydrolysis. Kinetics constants, Km and Vmax were estimated and were found to be affected by the bead size. The catalytic activity of the enzyme was studied in presence of various starchy residues and metal ions. HgCl2, CuSO4 and FeCl3 caused inhibition of the enzyme. The reaction conditions, pH and temperature, was optimized using response surface methodology. At the optimum pH and temperature of 4.9 and 57ºC, the apparent activity was 25.6U/g of beads, resulting in almost 2-fold increase in activity. The immobilized...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus circulans GRS313; Entrapment; Maltooligosaccharide-forming amylase; Response surface methodology; Starchy residues.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200005
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Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes BABT
Kumari,Annapurna; Mahapatra,Paramita; Banerjee,Rintu.
Optimization of lipase production by Enterobacter aerogenes was carried out using response surface methodology (RSM) where the statistical model was obtained by fractional factorial central composite design. The influence of various physico-chemical parameters, viz. temperature, oil concentration, inoculum volume, pH and incubation period on lipase production was examined. Optimization of physico-chemical parameters resulted 1.4- fold increase in lipase activity. The optimum levels of parameters were 34°C, oil concentration 3%, inoculum volume 7%, pH 7 and incubation time 60 h for obtaining a maximum lipase activity of 27.25 U/ml.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Central composite design; Enterobacter aerogenes; Lipase; Optimization; Parameter; Response Surface Methodology.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600005
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Isolation and characterization of a newly isolated Pseudomonas mutant for protease production BABT
Dutta,Jayati Ray; Banerjee,Rintu.
A potent bacterium for extracellular protease production was isolated from local soil and identified as Pseudomonas sp. RAJR 044. A mutant of this strain JNGR 242 with protease productivity 2.5 fold higher was obtained by ultraviolet irradiation under experimentally optimized conditions of pH 7.0, temperature of 34ºC, inoculum volume of 1.0 mL and incubation time of 24 hours. Comparative analysis of the chemical characteristics i.e. assimilation of carbon and nitrogen sources were also carried out. Maximum growth of the mutant strain in 2% gelatin agar plate was obtained in presence of dextrose (2%), maltose (2%), ammonium sulfate (2%) and potassium nitrate (2%) whereas, that of the parent strain was found in sucrose (2%) and ammonium nitrate (2%). The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular protease; Purification; Kinetic characterization; Ultraviolet mutagenesis; Pseudomonas sp.; Physicochemical.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100005
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Modeling gallic acid production rate by empirical and statistical analysis BABT
Kar,Bratati; Banerjee,Rintu; Bhattacharyya,Bimal Chandra.
For predicting the rate of enzymatic reaction empirical correlation based on the experimental results obtained under various operating conditions have been developed. Models represent both the activation as well as deactivation conditions of enzymatic hydrolysis and the results have been analyzed by analysis of variance (ANOVA). The tannase activity was found maximum at incubation time 5 min, reaction temperature 40ºC, pH 4.0, initial enzyme concentration 0.12 v/v, initial substrate concentration 0.42 mg/ml, ionic strength 0.2 M and under these optimal conditions, the maximum rate of gallic acid production was 33.49 mumoles/ml/min.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gallic acid; Modeling; Tannase; Caesalpinia digyna.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500010
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Growth characteristics modeling of Bifidobacterium bifidum using RSM and ANN BABT
Meena,Ganga S.; Gupta,Suneel; Majumdar,Gautam C.; Banerjee,Rintu.
The aim of this work was to optimize the biomass production by Bifidobacterium bifidum 255 using the response surface methodology (RSM) and artificial neural network (ANN) both coupled with GA. To develop the empirical model for the yield of probiotic bacteria, additional carbon and nitrogen content, inoculum size, age, temperature and pH were selected as the parameters. Models were developed using ¼ fractional factorial design (FFD) of the experiments with the selected parameters. The normalized percentage mean squared error obtained from the ANN and RSM models were 0.05 and 0.1%, respectively. Regression coefficient (R²) of the ANN model showed higher prediction accuracy compared to that of the RSM model. The empirical yield model (for both ANN and RSM)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Probiotics; Response surface methodology (RSM); FFD; Artificial neural network (ANN); Genetic algorithms (GA).
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600023
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Growth characteristics modeling of Lactobacillus acidophilus using RSM and ANN BABT
Meena,Ganga Sahay; Kumar,Nitin; Majumdar,Gautam Chandra; Banerjee,Rintu; Meena,Pankaj Kumar; Yadav,Vijesh.
The culture conditions viz. additional carbon and nitrogen content, inoculum size, age, temperature and pH of Lactobacillus acidophilus were optimized using response surface methodology (RSM) and artificial neural network (ANN). Kinetic growth models were fitted to cultivations from a Box-Behnken Design (BBD) design experiments for different variables. This concept of combining the optimization and modeling presented different optimal conditions for L. acidophilus growth from their original optimization study. Through these statistical tools, the product yield (cell mass) of L. acidophilus was increased. Regression coefficients (R²) of both the statistical tools predicted that ANN was better than RSM and the regression equation was solved with the help of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Response surface methodology (RSM); Artificial neural network (ANN); Genetic algorithms (GA); Box-behnken besign (BBD).
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100003
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Application of decolourized and partially purified polygalacturonase and α-amylase in apple juice clarification BJM
Dey,Tapati Bhanja; Banerjee,Rintu.
Polygalacturonase and α-amylase play vital role in fruit juice industry. In the present study, polygalacturonase was produced by Aspergillus awamori Nakazawa MTCC 6652 utilizing apple pomace and mosambi orange (Citrus sinensis var mosambi) peels as solid substrate whereas, α-amylase was produced from A. oryzae (IFO-30103) using wheat bran by solid state fermentation (SSF) process. These carbohydrases were decolourized and purified 8.6-fold, 34.8-fold and 3.5-fold, respectively by activated charcoal powder in a single step with 65.1%, 69.8% and 60% recoveries, respectively. Apple juice was clarified by these decolourized and partially purified enzymes. In presence of 1% polygalacturonase from mosambi peels (9.87 U/mL) and 0.4% α-amylase (899 U/mL), maximum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polygalacturonase; Α-amylase; Activated charcoal; Decolourization; Apple juice clarification.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100014
Registros recuperados: 11
Primeira ... 1 ... Última
 

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