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| Lima,Juliana O.; Pereira,Jorge F.; Rincones,Johana; Barau,Joan G.; Araújo,Elza F.; Pereira,Gonçalo A.G.; Queiroz,Marisa V.. |
| This report describes the cloning, sequence and expression analysis of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene of Moniliophthora perniciosa, the most important pathogen of cocoa in Brazil. Southern blot analysis revealed the presence of a single copy of the GAPDH gene in the M. perniciosa genome (MpGAPDH). The complete MpGAPDH coding sequence contained 1,461 bp with eight introns that were conserved in the GAPDH genes of other basidiomycete species. The cis-elements in the promoter region of the MpGAPDH gene were similar to those of other basidiomycetes. Likewise, the MpGAPDH gene encoded a putative 339 amino acid protein that shared significant sequence similarity with other GAPDH proteins in fungi, plants, and metazoans. Phylogenetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glyceraldehyde-3-phosphate dehydrogenase gene; Moniliophthora perniciosa; Witches' broom. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200024 |
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