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Leptospira spp. infection in sheep herds in southeast Brazil J. Venom. Anim. Toxins incl. Trop. Dis.
Barbante,Priscila; Shimabukuro,Fabio H; Langoni,Helio; Richini-Pereira,Virgínia B; Lucheis,Simone B.
Background With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, São Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp.Results MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospirosis; Ovine; Serology; Culture; PCR.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200325
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