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Barbosa,Aneli M.; Souza,Cristina G. M.; Dekker,Robert F. H.; Fonseca,Rafael C.; Ferreira,Dalva T.. |
Four virulent strain isolates of the fungus, Bipolaris euphorbiae (previously identified as a Helminthosporium sp.), isolated from host plants in four states within Brazil were screened for the production of phytotoxins that promoted wilting and defoliation of the Brazilian weed, Euphorbia heterophylla, commonly found growing among soyabean crops. Only one isolate, B. euphorbiae Strain I (EUPH petropar from Mato Grosso state), produced phytotoxin in-vitro when grown in stationary culture for 7 d at 28 ° C on minimum salts medium supplemented with 1.5 % glucose as the sole carbon source. Phytotoxin was also produced when the fungal strain was grown on fructose, galactose, mannose, xylose and sucrose. The addition of nitrogen source (yeast extract, peptone... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bipolaris euphorbiae; Euphorbia heterophylla; Phytotoxin production; Phytotoxin stability. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200016 |
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Saldanha,Roze L.; Garcia,José E.; Dekker,Robert F. H.; Vilas-Boas,Laurival A.; Barbosa,Aneli M.. |
Nine isolates of Botryosphaeria spp. were evaluated for their growth and the production of cell wall-lytic enzymes (laccase, pectinase and beta-1,3-glucanase) when grown on basal medium in the absence and presence of the laccase inducer, veratryl alcohol (VA). The genetic relationship among the nine isolates collected from different host plants was determined by RAPD analyses. ITS sequence analysis showed eight closely related isolates classified as Botryosphaeria rhodina, and one isolate classified as Botryosphaeria ribis. RAPD analysis resolved the isolates into three main clusters based upon levels of laccase and beta-1,3-glucanase activity. There appears to be no correlation between pectinase production and genetic diversity among the nine isolates.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Botryosphaeria isolates ITS and RAPD Laccase Pectinase beta-1; 3-Glucanase. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200013 |
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