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Biofilm formation by Staphylococcus aureus from food contact surfaces in a meat-based broth and sensitivity to sanitizers BJM
Souza,Evandro Leite de; Meira,Quênia Gramile Silva; Barbosa,Isabella de Medeiros; Athayde,Ana Júlia Alves Aguiar; Conceição,Maria Lúcia da; Siqueira Júnior,José Pinto de.
This study assessed the capacity of adhesion, the detachment kinetic and the biofilm formation by Staphylococcus aureus isolated from food services on stainless steel and polypropylene surfaces (2 x 2 cm) when cultivated in a meat-based broth at 28 and 7 ºC. It was also to study the efficacy of the sanitizers sodium hypochlorite (250 mg/L) and peracetic acid (30 mg/L) in inactivating the bacterial cells in the preformed biofilm. S. aureus strains adhered in high numbers regardless the assayed surface kind and incubation temperature over 72 h. Cells detachment of surfaces revealed high persistence over the incubation period. Number of cells needed for biofilm formation was noted at all experimental systems already after 3 days. Peracetic acid and sodium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Food processing environment; Cross-contamination; Biofilm; Sanitizers.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100010
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Adhesion and biofilm formation by Staphylococcus aureus from food processing plants as affected by growth medium, surface type and incubation temperature BJPS
Jerônimo,Heloísa Maria Ângelo; Queiroga,Rita de Cássia Ramos do Egypto; Costa,Ana Caroliny Vieira da; Barbosa,Isabella de Medeiros; Conceição,Maria Lúcia da; Souza,Evandro Leite de.
This study assessed the effect of different growth media [BHI broth, BHI broth plus glucose (10 g/100 mL) and BHI broth plus NaCl (5 g/100 mL)] and incubation temperatures (28 or 37 ºC) on the adherence, detachment and biofilm formation on polypropylene and stainless steel surfaces (2 x 2 cm coupons) for a prolonged period (24-72 h) by some strains of Staphylococcus aureus (S3, S28 and S54) from food processing plants. The efficacy of the sanitizers sodium hypochlorite (250 mg/mL) and peracetic acid (30 mg/mL) in reducing the number of viable bacterial cells in a preformed biofilm was also evaluated. S. aureus strains adhered in highest numbers in BHI broth, regardless of the type of surface or incubation temperature. Cell detachment from surfaces revealed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus/food processing; Biofilm; Surfaces; Sanitizers/efficacy.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400018
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