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Detoxification of Pacific oyster Crassostrea gigas fed on diets of Skeletonema costatum with and without silt, following PSP contamination by Alexandrium minutum ArchiMer
Gueguen, Marielle; Bardouil, Michele; Baron, Regis; Lassus, Patrick; Truquet, Philippe; Massardier, Julie; Amzil, Zouher.
Contamination of shellfish by paralytic shellfish poisoning ( PSP) toxins poses an economic threat to shellfish farmers. As contaminated shellfish cannot be harvested for long periods of time, it would be very useful to develop processes to optimise and shorten their detoxification. In this study, Pacific oysters Crassostrea gigas were first experimentally contaminated over a period of 13 days with a continuous flow of toxic Alexandrium minutum cultures at concentrations ranging from 150 to 200 cell ml(-1) ( toxin content after 13 days of contamination 438 mu g STX equiv. 100 g(-1) wet weight). Then, two different detoxification treatments were tested and showed detoxification rates greater than those observed in coastal environments. The first treatment...
Tipo: Text Palavras-chave: Alexandrium minutum; Crassostrea gigas; Detoxification; Inorganic matter; Organic matter; PSP; Paralytic shellfish poisoning.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3896.pdf
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Numerical and experimental study of a continuous electrostatic smoking process ArchiMer
Baron, Regis; Havet, Michel; Solliec, Camille; Pierrat, Daniel; Touchard, Gerard.
The smoking process is a batch one with long duration processing (some hours). Deposition of smoke particles can be enhanced by electrostatic precipitation. However, a better knowledge of the fundamental physics is needed to optimize the process. We carried out experimental and numerical studies to obtain essential information on the smoke flow. First, experiments physically characterized the wood smoke. These measures lead us to study the turbulent two-phase flow using an algebraic slip model. The main coupling with electrical forces is taken into account through the slip velocity. Various assumptions were tested concerning the coupled phenomena in order to build an algorithm that is able to simulate these phenomena. Numerical resolutions are compared...
Tipo: Text Palavras-chave: Smoked salmon; Smoke particles; Electrostatic precipitators.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4695.pdf
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First insight on interactions between bacteria and the marine diatom Haslea ostrearia : Algal growth and metabolomic fingerprinting ArchiMer
Lepinay, Alexandra; Turpin, Vincent; Mondeguer, Florence; Grandet-marchant, Quentin; Capiaux, Herve; Baron, Regis; Lebeau, Thierry.
A first insight on the interactions between bacteria isolated from monospecific cultures of the marine diatom Haslea ostrearia and this microalgae is described in this work. From 28 bacterial isolates, the maximal growth rate and the maximal cell concentration were on average 58% and 27% higher (max. 67% for isolate B22). Isolates B5, B10 (both Saccharospirillum sp.) and B22 (Rhodobacter sp.) were the most efficient. The geographical origin of H. ostrearia from which bacteria were isolated had no impact on the algal growth performances, and a given bacterial isolate led to the same algal growth irrespective of the geographical origin of H. ostrearia. To explain the results obtained, a comprehensive integrative strategy was conducted that analyzed the...
Tipo: Text Palavras-chave: Bacterial selection; Plant-growth-promoting bacteria; Phycosphere; Metabolomics.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00429/54036/55340.pdf
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A Physiologically Structured Equation to Consider Quota Heterogeneity in the Droop Model ArchiMer
Mairet, Francis; Baron, Regis.
The Droop model allows to represent microalgae growth limited by a nutrient, using a cell quota (also referred to as variable-yield) approach. Single-cell measurements have revealed quota heterogeneity in phytoplankton collected from field studies. Such heterogeneity can be due, among other factors, to spatial structure (e.g. in biogeochemical cycles in the ocean, or for photobioreactors connected in series). Nonetheless, quota heterogeneity is generally omitted in modelling studies, using an average quota approach, or included in size-structured or individual-based models. Here, we propose a distributed Droop equation to tackle this problem, considering subpopulation growth -in line with Droop macroscopic view- rather than cell division dynamics. We...
Tipo: Text Palavras-chave: Population Balance Model; Variable-yield model; Cell Quota; Phytoplankton; Microalgae.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00600/71187/69542.pdf
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Manufacture of "fresh/wet" aquaculture feeds based on by-catches, fish by-products and algae ArchiMer
Doudard, Karine; Chim, Liet; Arhaliass, Abdellah; Przybyla, Cyrille; Baron, Regis.
This work focuses on available bio resources transformation such as by-catches or fish by-products and marine plants (seaweed and microalgae) as ren circuit . The objective is to make this production as autonomous and sustainable as possible and fitted to the idea of circular economy. Thanks to previous projects, it seems that by-catches could be a plentiful source of proteins for fish. The challenge associated with this type of aquaculture formulations relies on the ability to incorporate abundant resources readily available and the significant reduction of certain energy‐consuming steps (such as drying) in order to minimize the environmental impact of a fish feed production. The adequacy of this strategy (fresh raw material and wet feed) with a...
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Ano: 2018 URL: http://archimer.ifremer.fr/doc/00456/56736/58525.pdf
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Odor Modification in Salmon Hydrolysates Using the Maillard Reaction ArchiMer
Kouakou, Christelle; Berge, Jean-pascal; Baron, Regis; Lethuaut, Laurent; Prost, Carole; Cardinal, Mireille.
The aim of this work was to study the effect of adding sugar during proteolysis to promote the Maillard reaction and mask the initial fish odor and off-flavors generated. An experimental design, based on the Doehlert plan, was used to study the influence of hydrolysis conditions (time, temperature, sugar, and antioxidant addition) on the odor characteristics of hydrolysates, soluble protein levels, and amino acid content. Results showed that the lowest level of sugar (10g of D-xylose added to 1kg of by-products) was enough to develop a grilled odor in hydrolysates. In the hydrolysis conditions used, i.e. enzyme inactivation at 95°C for 30min, hydrolysis temperature had no effect on grilled odor production but significantly affected the soluble protein...
Tipo: Text Palavras-chave: Salmon by-product; Hydrolysate; Maillard reaction; Sensory characteristics; Off-flavor; Soluble protein.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00156/26702/24778.pdf
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Etude numérique de la répartition de fumée dans une enceinte de fumage industrielle. Rapport final ArchiMer
Havet, Michel; Baron, Regis; Le Niliot, Anne.
Le laboratoire Génie Alimentaire de l'IFREMER travaille depuis quelques années sur le procédé de fumage en utilisant1 une enceinte climatisée industrielle. Pour optimiser ce procédé afin d'en tirer le meilleur partie pour nos différentes recherches, il est apparu indispensable de modéliser et simuler l'écoulement des particules de fumée. Ce travail nécessite, à la fois, un recherche bibliographique critique très pointue et un travail de simulation avec des outils logiciels (code de calcul CFD) sophistiqués. Ne disposant pas des moyens nécessaires en interne, le laboratoire a donc choisi de contractualisé cette tâche avec l'ENITIAA.
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Ano: 2002 URL: http://archimer.ifremer.fr/doc/00134/24521/22550.pdf
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Improving detoxification Efficiency of PSP-contaminated oysters (Crassostrea gigas Thunberg) ArchiMer
Lassus, Patrick; Bardouil, Michele; Baron, Regis; Berard, Jean-baptiste; Masselin, Pierre; Truquet, Philippe; Pitrat, Jp.
Shellfish production, and production of marine bivalve molluscs in particular, is the main form of marine aquaculture in the European Union, with a production estimated at 1,200,000 tons in 1999 (Food and Agricultural Organization). Moreover, aquaculture production is rapidly expanding throughout Europe and is becoming a major economic resource in developing areas such as Northern Greece, Ireland, and northwestern Spain. These shellfish, mainly mussels, oysters, and clams, are produced in a natural environment. Due to variations in their environment, these otherwise edible species may however become unsafe for human consumption. Potential problems are amplified due to the nature of shellfish themselves: as they are filter feeders, they consume large...
Tipo: Text Palavras-chave: Detoxification; PSP toxin; Toxic algae; Bioaccumulation; Contaminant; Clam; Mussel; Oyster; Shellfish culture.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-2271.pdf
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Comparative Value of a Sorting Procedure and Quantitative Descriptive Analysis to Investigate the Influence of Processing Parameters: Case Study of Hydrolysate Production From Salmon By-Products ArchiMer
Cardinal, Mireille; Baron, Regis; Kouakou, Christelle; Prost, Carole; Courcoux, Philippe.
Many papers have recently discussed the value of a free sorting method as a rapid and simple alternative to quantitative descriptive analysis, considered the reference tool for food sensorial characterization. The aim of the present paper is to evaluate whether this method of free sorting can also be used to investigate the influence of processing parameters. An experimental design was applied to production conditions of enzymatic hydrolysates from salmon by-products. The effect of four processing parameters (time and temperature of hydrolysis, sugar and antioxidant addition) on the odor of the hydrolysates was studied using a sorting task with 45 untrained panelists and a quantitative descriptive analysis carried out with 11 trained panelists. This study...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00179/29051/27488.pdf
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Gut passage times in two bivalve molluscs fed toxic microalgae: Alexandrium minutum, A-catenella and Pseudo-nitzschia calliantha ArchiMer
Gueguen, Marielle; Lassus, Patrick; Laabir, Mohamed; Bardouil, Michele; Baron, Regis; Sechet, Veronique; Truquet, Philippe; Amzil, Zouher; Barille, Laurent.
The occurrence of new phytoplankton species in a coastal area may be explained by the import of shellfish containing whole live algal cells in their digestive tracts. Indeed, shellfish containing toxic algal cells can induce both primary contaminations in safe areas ( initially free from toxic microalgae), and secondary contaminations of other shellfish growing in the same area via the faeces of the imported animals. To mitigate this problem, shellfish need to be placed in a separate holding tank and their intestinal content purged. For a deeper understanding of the risks associated with transferring contaminated shellfish, oysters ( Crassostrea gigas) and mussels ( Mytilus edulis) were purposely fed either Alexandrium minutum or A. catenella (...
Tipo: Text Palavras-chave: Risk assessment; Toxic phytoplankton; Uptake; Gut passage; Mytilus edulis; Crassostrea gigas.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3897.pdf
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Kinetic study of solid phase demineralization by weak acids in one-step enzymatic bio-refinery of shrimp cuticles ArchiMer
Baron, Regis; Socol, Marius; Arhaliass, A.; Bruzac, Sandrine; Le Roux, Karine; Del Pino, J. Rodriguez; Berge, Jean-pascal; Kaas, Raymond.
We describe a one-step bio-refinery process for shrimp composites by-products. Its originality lies in a simple rapid (6 h) biotechnological cuticle fragmentation process that recovers all major compounds (chitins, peptides and minerals in particular calcium). The process consists of a controlled exogenous enzymatic proteolysis in a food-grade acidic medium allowing chitin purification (solid phase), and recovery of peptides and minerals (liquid phase). At a pH of between 3.5 and 4, protease activity is effective, and peptides are preserved. Solid phase demineralization kinetics were followed for phosphoric, hydrochloric, acetic, formic and citric acids with pKa ranging from 2.1 to 4.76. Formic acid met the initial aim of (i) 99 % of demineralization yield...
Tipo: Text Palavras-chave: Bio-refinery; Acidic enzymatic proteolysis; Shrimp cuticles; Demineralization kinetics and modeling.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00281/39244/37809.pdf
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Procédé d'extrusion réactive et/ou enzymatique pour l'extraction de phycocolloïdes : application aux produits de la mer ArchiMer
Makoure, Dounia; Arhaliass, Abdellah; Echchelh, Adil; Baron, Regis; Legrand, Jack.
In this paper we present the reactive extrusion process to extract the phycocolloids from the sea products. By comparing with other techniques, this process has a several advantages like the economic return, the flexibility, the production of various products with different characteristics and time gains. There are two types of extruder: single-screw and double-screw, each one has its specific properties. The solid products are introduced by means of the hopper that is equipped with a dosage pump for the regulation of the flow. Liquid products are, generally, injected in the not filled zones, through an external pump. The products undergo thermo mechanical modifications and then the shaping of the final products was done in the die. The reactive extrusion...
Tipo: Text Palavras-chave: Extrusion réactive; Valorisation; Coproduits; Produits de la mer; Phycocolloïdes alginate; Reactive extrusion; Valorization; By-products; Sea-products; Phycocolloids; Alginate.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00500/61171/64645.pdf
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Modélisation de la contamination des bivalves par les toxines d'Alexandrium spp. Rapport final ArchiMer
Lassus, Patrick; Amzil, Zouher; Bardouil, Michele; Baron, Regis; Bohec, Madeleine; Bougrier, Serge; Garen, Pierre; Laabir, Mohamed; Leguay, Didier; Masselin, Pierre; Truquet, Philippe; Sechet, Veronique; Bablon, Clotilde; Berard, Jean-baptiste; Carre, Camille; Cheignon, Valerie; Lecamp, Sylvain; Mabit, Marie.
L'objectif général de l'étude est de simuler les épisodes de contamination de certains bivalves (huîtres et moules) par les toxines d'Alexandrium minutum et A. calenella à partir d'un module expérimental adapté, et en vue de construire un modèle de contamination applicable à ces espèces. Ce modèle sera ensuite susceptible d'être couplé aux modèles biologiques locaux (estuaire de Penzé et étang de Thau) de déterminisme des blooms, ainsi qu'aux modèles hydrodynamiques développés par différentes équipes (Ifremer, Université de Montpellier). La première année (2001-2002) était consacrée à la simulation des contaminations en estuaire de Penzé (Alexandrium minulum) et au développement de cultures en masse d'A. calenella de Thau en bioréacteurs.
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Ano: 2002 URL: http://archimer.ifremer.fr/doc/00156/26711/24786.pdf
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Modélisation de la contamination des huîtres par les toxines d'Alexandrium minutum - Rapport final (2ème année) ArchiMer
Lassus, Patrick; Bardouil, Michele; Baron, Regis; Garen, Pierre; Laabir, Mohamed; Leguay, Didier; Masselin, Pierre; Truquet, Philippe; Joret, Camille.
L'objectif de l'étude est de simuler les épisodes de contamination des huîtres par les toxines d'Alexandrium minufum à partir d'un module expérimental adapté, et en vue de construire un modèle de contamination applicable à cette espèce. Ce modèle sera ensuite susceptible d'être couplé au modèle biologique local (estuaire de Penzé) de déterminisme des blooms, ainsi qu'aux modèles hydrodynamiques développés par différentes équipes (Ifremer).
Tipo: Text
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00156/26712/24787.pdf
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Optimisation d'un nouveau procédé de fumage du poisson par précipitation électrostatique des particules de fumée ArchiMer
Baron, Regis; Sanquer, Odile; Département Sciences et Techniques Alimentaires Marines de l'IFREMER de Nantes; UMR CNRS GEPEA; CETIM; UMR LEA Univ. Poitiers.
Cette étude porte sur l'optimisation d'un nouveau procédé de fumage en continu par précipitation électrostatique des particules de fumée. Le principe est de faire circuler Ia fumée, produite par pyrolyse de bois, entre deux électrodes. Sous des conditions électriques spécifiques, un flux ionique entre les électrodes est généré.
Tipo: Text
Ano: 2006 URL: http://archimer.ifremer.fr/doc/00065/17626/15155.pdf
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Utilisation des sous-produits de la pêche et de l'aquaculture pour l'alimentation en aquaculture ArchiMer
Penven, Anais; Baron, Regis; Etienne, Monique; Delannoy, Charles; Berge, Jean-pascal.
Tipo: Text
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00607/71938/70635.pdf
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Soft liquefaction of the red seaweed Grateloupia turuturu Yamada by ultrasound-assisted enzymatic hydrolysis process ArchiMer
Le Guillard, Cecile; Berge, Jean-pascal; Donnay-moreno, Claire; Bruzac, Sandrine; Ragon, Jean-yves; Baron, Regis; Fleurence, Joel; Dumay, Justine.
Ultrasound-assisted enzymatic hydrolysis is a recent process, increasingly employed for plant biomass liquefaction and the recovery of soluble biomolecules. However, to our knowledge, it has never been used on seaweeds, particularly wet ones. The aim of this study was to compare the efficiency of three processes on the liquefaction of the red seaweed Grateloupia turuturu Yamada: enzyme-assisted extraction (EAE), ultrasound-assisted extraction (UAE), and their combination, ultrasound-assisted enzymatic hydrolysis (UAEH). These comparisons will allow the identification as to which process achieves the highest extraction yield of water-soluble compounds. For this purpose, experiments were conducted at 40 °C for 6 h using an enzymatic cocktail of four...
Tipo: Text Palavras-chave: Ultrasound-assisted enzymatic hydrolysis; Enzymatic hydrolysis; Ultrasound; Liquefaction; Algae; Grateloupia turuturu.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00308/41877/44316.pdf
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A New Process for Extracting Alginates from Laminaria digitata: Reactive Extrusion ArchiMer
Vauchel, Peggy; Kaas, Raymond; Arhaliass, Abdellah; Baron, Regis; Legrand, Julien.
Alginates are natural polysaccharides that are extracted from brown seaweeds and widely used for their rheological properties. The main step in the extraction protocol used in the alginate industry is that of alkaline extraction. A batch process is used for this step, which is time-, water-, and reactant-consuming. The possibility of extracting by reactive extrusion was investigated. The reactive extrusion process appeared to be more efficient than the batch process for the alkaline extraction of alginates from Laminaria digitata in several key ways: Time demand is reduced from about an hour to only few minutes, water and reactant requirements are divided by more than a factor 2, extraction yield is 15% higher (relative enhancement), and the rheological...
Tipo: Text Palavras-chave: Intrinsic viscosity; Reactive extrusion; Laminaria digitata; Alkaline extraction; Alginate.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6255.pdf
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Transient initial phase in continuous culture of Isochrysis galbana affinis Tahiti ArchiMer
Bougaran, Gael; Le Dean, Loic; Lukomska, Ewa; Kaas, Raymond; Baron, Regis.
Starting microalgae continuous culture generally includes a preliminary batch culture to obtain sufficient cell density. It is possible to apply continuous regime from the beginning of the culture (IC mode) rather than to begin by an initial batch (IB mode). It is our purpose to check that both initial modes lead to identical steady-state cell characteristics. The microalga Isochrysis galbana affinis Tahiti was used for this comparative study. With an initial cell density of 5 x 10(5) cell ml(-1) and a dilution rate of about 1.0 d(-1), both IB and IC modes led to identical cell density once steady-state is reached between 6 and 8 d after inoculation in the two cases. Cell concentration of chlorophyll a and pheopigment a were found to be similar for IB and...
Tipo: Text Palavras-chave: Isochrysis; Cell quota; Photosynthesis; Steady state; Continuous culture.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-597.pdf
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Effects of the smoking process on odour characteristics of smoked herring (Clupea harengus) and relationships with phenolic compound content ArchiMer
Cardinal, Mireille; Cornet, Josiane; Serot, Thierry; Baron, Regis.
The relationship between smoking parameters and odour characteristics, evaluated by a trained sensory panel.. were studied on smoked herring. In addition, a possible correlation between the content of 10 phenolic compounds and sensory perceptions was investigated. Five smoking techniques were applied, combining smoke production conditions, performed by pyrolysis of beech wood sawdust or by friction of beech wood log, with smoke deposition, either in a controlled kiln (traditional smoking) or by an electrostatic process. In the fifth smoking technique, a purified condensate of beech smokes was vaporised on fish fillets in the smokehouse. The time of smoking was 3 It for traditional smoking and the liquid smoke atomisation process and 12 min for the...
Tipo: Text Palavras-chave: Odour characteristics; Sensory evaluation; Phenolic compounds; Smoking; Herring.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-827.pdf
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