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A method to assess algicidal activity of microalgal extracts coupling microalgae produced in stirred closed photobioreactor operating in continuous with pulse amplitude modulated (PAM) fluorometry ArchiMer
Menguy, Eva; Dumontet, Vincent; Coulombier, Noémie; Meriot, Vincent; Le Dean, Loic; Barthelemy, Vanille; Jauffrais, Thierry.
We describe in the present study a quick and reliable method based on chlorophyll a fluorescence to assess putative algicidal effect of different microalgal extracts. We couple microalgal production under chemostat cultivation mode to continuously produce a given microalgae species (e.g. Dunaliella salina in this study) at a stable physiological state to ease comparison between extracts tested; with a non-destructive method based on chlorophyll a fluorescence. Pulse amplitude modulated (PAM) fluorometry was used to assess over time the effect of different microalgal crude extracts on the efficiency of the photosystem II (PSII) of a tested microalgae (Dunaliella salina). • Microalgal production at stationary phase in stirred closed photobioreactor (PBR)...
Tipo: Text Palavras-chave: Bioassay; Cytoxicity; Microalgae; Quantum efficiency; Fv/Fm; Non photochemical quenching; NPQ; Electron transport rate; Phytochemistry; Anti algal.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00643/75524/76400.pdf
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Acquisition de données complémentaires aux dénombrements, avec les techniques de cytométrie en flux, fluorescence totale ou spectrale. Etat d’avancement et premiers résultats. Action Indice Composition. Livrable n° A III. Rapport final, 23 septembre 2014 ArchiMer
Artigas, Luis Felipe; Didry, Morgane; Barthelemy, Vanille; Bonato, Simon; Broutin, Mathias; Lizon, Fabrice; Lefebvre, Alain.
The European Union Water Framework Directive (WFD) refers to phytoplankton as one of the biological quality elements that should be regularly monitored and evaluated thanks to three ecological indices: biomass, abundance and composition. The first two have already been defined, but the third one has not yet been proposed in the English Channel-Atlantic zone. The construction of a composition indice must consider the diversity of all the phytoplankton. As the microscopic counts concern only the micro-phytoplankton, it is necessary to use additional information which concerns the nano- and pico-phytoplankton, of great importance when estimating the phytoplankton diversity. These components of the phytoplankton can be measured by different complementary...
Tipo: Text Palavras-chave: Manche Orientale; Groupes phytoplanctoniques; Cytométrie en Flux de type Scanning; Fluorimétrie spectrale; Analyses pigmentaires; Eastern English Channel; Phytoplankton groups; Scanning Flow Cytometry; Spectral fluorescence; Pigments analysis.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00361/47259/47224.pdf
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Effects of Nitrogen Availability on the Antioxidant Activity and Carotenoid Content of the Microalgae Nephroselmis sp ArchiMer
Coulombier, Noémie; Nicolau, Elodie; Le Déan, Loic; Barthelemy, Vanille; Schreiber, Nathalie; Brun, Pierre; Lebouvier, Nicolas; Jauffrais, Thierry.
Nephroselmis sp. was previously identified as a species of interest for its antioxidant properties owing to its high carotenoid content. In addition, nitrogen availability can impact biomass and specific metabolites’ production of microalgae. To optimize parameters of antioxidant production, Nephroselmis sp. was cultivated in batch and continuous culture conditions in stirred closed photobioreactors under different nitrogen conditions (N-repletion, N-limitation, and N-starvation). The aim was to determine the influence of nitrogen availability on the peroxyl radical scavenging activity (oxygen radical absorbance capacity (ORAC) assay) and carotenoid content of Nephroselmis sp. Pigment analysis revealed a specific and unusual photosynthetic system with...
Tipo: Text Palavras-chave: Lutein; Natural products; Nutrients; Peroxyl radical; Siphonaxanthin.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00645/75699/76604.pdf
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