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| Santos,Roberta Tancredi Francesco dos; Silva,Marcelo Florencio Passos; Porto,Rafael Marques; Lebrun,Ivo; Gonçalves,Luís Roberto de Camargo; Batista,Isabel de Fátima Correia; Sandoval,Maria Regina Lopes; Abdalla,Fernando Maurício Francis. |
| Abstract Background: Here, we described the presence of a neurotoxin with phospholipase A2 activity isolated from Micrurus lemniscatus venom (Mlx-8) with affinity for muscarinic acetylcholine receptors (mAChRs). Methods: The purification, molecular mass determination, partial amino acid sequencing, phospholipase A2 activity determination, inhibition of the binding of the selective muscarinic ligand [3H]QNB and inhibition of the total [3H]inositol phosphate accumulation in rat hippocampus of the Mlx-8 were determined. Results: Thirty-one fractions were collected from HPLC chromatography, and the Mlx-8 toxin was used in this work. The molecular mass of Mlx-8 is 13.628 Da. Edman degradation yielded the following sequence:... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Muscarinic receptors; Hippocampus; Micrurus lemniscatus; Inositol phosphate; Phopholipase A2. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100301 |
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