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| Possamai,Mari Helen Pagani; Battilana,Jaqueline; Paludo,Ediane; Herkenhoff,Marcos Edgar; Pértile,Fábio; Lima-Rosa,Carlos André da Veiga. |
| This study aimed to investigate the genetic variability of two Brazilian free range (Caipira) chickens lines using microsatellites analysis of ten loci. It was collected a total of 99 blood samples, which 49 were from Paraíso Pedrês (PP) and 50 were from Rubro Negra (RN) lines. The amplification of the DNA fragments was performed by polymerase chain reaction (PCR) and the genotyping was conduct using ABI 3130 sequencer. The allele number variation was among 3 (LEI0254) to 32 (LEI0212) in the PP line, and 4 (LEI0254) to 31 (LEI0212) in the RN line. The allelic average per locus was 13.3 and 13.1 in the PP and RN lines, respectively. The average observed and the expected heterozygosity were 0.650 and 0.820 in the PP line, and 0.671 and 0.804 in the RN line.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: (Caipira) broiler line; (Caipira) layer line; Microsatellite; Polymorphism. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500877 |
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