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| Mosbah, Amor; Delavenne, Emilie; Souissi, Yasmine; Mahjoubi, Mouna; Jehan, Philippe; Le Yondre, Nicolas; Cherif, Ameur; Bondon, Arnaud; Mounier, Jerome; Baudy-floc'H, Michele; Le Blay, Gwenaelle. |
| Lactobacillus harbinensis K.V9.3.1Np was described as endowed with high antifungal activity. Most of the studies associated this activity to the produced organic acids, i.e., lactic acid, acetic acid, and hexanoic acid. The aim of this study was to purify and identify, other not yet described, antifungal molecules produced by L. harbinensis K.V9.3.1 Np when used in yogurt fermentation. Active compounds were extracted through several extraction processes using organic solvents and protein precipitation. The fractions of interest were purified using flash chromatography and preparative HPLC for specific characterization. The bioactive compounds identification was performed using Nuclear Magnetic Resonance and Mass Spectrometry. Activity tests against... |
| Tipo: Text |
Palavras-chave: Spermine; Polylactates; Lactobacillus; Extraction; Purification; Ms; Nmr. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00616/72773/72221.pdf |
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| Bernay, Benoît; Baudy-floc'H, Michele; Zanuttini, Bruno; Zatylny, Céline; Pouvreau, Stephane; Henry, Joël. |
| For more than six decades, several studies have shown that genital products to entering the mantle cavity via the incurrent siphon, initiate in oyster, strong and rhythmic contractions of the adductor muscle (AM). In order to characterize the regulatory peptides capable of triggering AM contractions, we focused on the identification of putative myotropic peptides from genital products. Two experimental approaches were developed. The first one, based on a mass spectrometry screening of the male genital products, led to the identification of the tetrapeptide APGWamide. This neuropeptide was also detected in the seminal secretions of the cephalopod Sepia officinalis. In this species, APGWamide is directly involved in the oocyte transport. In Crassostrea, in... |
| Tipo: Text |
Palavras-chave: Mass spectrometry; Myotropin; APGWamide; Egg laying; Molluscan. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1697.pdf |
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