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Pore-linked filaments in anura spermatocyte nuclei Anais da ABC (AABC)
Beçak,Maria Luiza; Fukuda-Pizzocaro,Kazumi.
Pore-linked filaments were visualized in spreads of anuran spermatocyte nuclei using transmission electron microscope. We used Odontophrynus diplo and tetraploid species having the tetraploid frogs reduced metabolic activities. The filaments with 20-40 nm width are connected to a ring component of the nuclear pore complex with 90-120 nm and extend up to 1µm (or more) into the nucleus. The filaments are curved and connect single or neighboring pores. The intranuclear filaments are associated with chromatin fibers and related to RNP particles of 20-25 nm and spheroidal structures of 0.5µm, with variations. The aggregates of several neighboring pores with the filaments are more commonly observed in 4n nuclei. We concluded that the intranuclear filaments may...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pore-linked filaments; Intranuclear filaments; Clustered-like NPCs in polyploid anurans; RNA transport.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000100009
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Somatic pairing, endomitosis and chromosome aberrations in snakes (Viperidae and Colubridae) Anais da ABC (AABC)
Beçak,Maria Luiza; Beçak,Willy; Pereira,Alexandre.
The positioning of macrochromosomes of Bothrops jararaca and Bothrops insularis (Viperidae) was studied in undistorted radial metaphases of uncultured cells (spermatogonia and oogonia) not subjected to spindle inhibitors. Colchicinized metaphases from uncultured (spleen and intestine) and cultured tissues (blood) were also analyzed. We report two antagonic non-random chromosome arrangements in untreated premeiotic cells: the parallel configuration with homologue chromosomes associated side by side in the metaphase plate and the antiparallel configuration having homologue chromosomes with antipolar distribution in the metaphase ring. The antiparallel aspect also appeared in colchicinized cells. The spatial chromosome arrangement in both configurations is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Snakes; Somatic pairing; Chromosome positioning; Endomitosis; Diplochromosomes.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652003000300004
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