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| Louveaux, Marion; Rochette, Sebastien; Beauzamy, Lena; Boudaoud, Arezki; Hamant, Olivier. |
| Exogenous mechanical perturbations on living tissues are commonly used to investigate whether cell effectors can respond to mechanical cues. However, in most of these experiments, the applied mechanical stress and/or the biological response are described only qualitatively. We developed a quantitative pipeline based on microindentation and image analysis to investigate the impact of a controlled and prolonged compression on microtubule behaviour in the Arabidopsis shoot apical meristem, using microtubule fluorescent marker lines. We found that a compressive stress, in the order of magnitude of turgor pressure, induced apparent microtubule bundling. Importantly, that response could be reversed several hours after the release of compression. Next, we tested... |
| Tipo: Text |
Palavras-chave: Mechanical stress; Indenter; Microtubules; Compression; Bundling; Arabidopsis thaliana; Technical advance. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00347/45829/45479.tiff |
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