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Internal control in PCR for Mycobacterium tuberculosis: usefulness and improvement of the diagnosis BABT
Cortez-Herrera,Elizabeth; Sperhacke,Rosa Dea; Becker,Daniela; Kritski,Afrânio; Zaha,Arnaldo; Rossetti,Maria Lucia Rosa.
The aim of this work was to construct and test a plasmidial Internal Control (IC) to detect the inhibition in the PCR test for M. tuberculosis and also its contribution for a Public Health Laboratory routine. The IC was a 600-bp of DNA linked to a plasmid with the same primer sites, allowing the amplification with the 245-bp diagnostic fragment. The amplification of the positive samples rendered the IC and the diagnostic fragment; instead negative samples only showed the IC. A total of 149 tuberculosis samples were studied and introduced the IC to monitor. Results showed 3.3% of the samples without amplification of the IC, suggesting the inhibition. These samples showed results in accordance with the clinical results. The objective of the IC was to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polymerase Chain Reaction; Tuberculosis; Internal control; Nucleic acid amplification; Uracyl DNA glycosilase.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400006
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