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Ledo,Ana da Silva; Lameira,Osmar Alves; Benbadis,Abdellatif Kemaleddine. |
Este trabalho objetivou avaliar as respostas morfogenéticas de diferentes explantes de cupuaçuzeiro (Theobroma grandiflorum Schum.) submetidos a várias condições de cultura in vitro. A utilização de 2,4-D nas concentrações de 4,52; 6,78 e 9,05 µM promoveu a formação de calos friáveis em eixos embrionários e o TDZ nas concentrações de 2,0 e 3,0 µM induziu uma alta frequência de calos friáveis e menor oxidação em segmentos de folhas jovens. O 2,4-D combinado com KIN, promoveu a iniciação de calos friáveis e o ANA combinado com KIN, a rizogênese em segmentos cotiledonares. Na presença de 2,26 µM de 2,4-D e 13,94 µM de KIN, houve a formação de calos friáveis em segmentos caulinares. A adição de água de coco, 2iP e ANA estimulou o rápido crescimento e a... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Theobroma grandiflorum; Calos; Reguladores de crescimento. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452002000300005 |
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Ledo,Ana da Silva; Lameira,Osmar Alves; Benbadis,Abdellatif Kemaleddine; Menezes,Ilmarina Campos de; Oliveira,Maria do Socorro Padilha de; Medeiros Filho,Sebastião. |
The aim of this work was to study the morphogenetic responses of zygotic embryos of açai palm (Euterpe oleracea Mart.) submitted to several conditions of in vitro culture. Several research experiments were conducted, in laboratory, using vegetable material collected from açai palm plants at Embrapa Amazon Oriental, Belém-PA, Brazil. It was possible to verify the expression of a direct, repetitive and no-synchronized model of somatic embryogenesis in mature zygotic embryos cultivated in primary MS medium supplemented with 2,4-D (339.36 muM) and transferred to a secondary MS medium in the presence of NAA (0.537 muM) and 2iP (12.30 muM). The conversion of somatic embryos into seedlings was reached after 210 days with the transfer of the cultures to a third... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Açai palm; Growth regulators; Micropropagation; Tissue culture. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452002000300004 |
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Carvalho,Cristina Paiva da Silveira; Correia,Diva; Benbadis,Abdellatif Kemaleddine; Luz,José Magno Queiroz; Rossetti,Adroaldo Guimarães. |
Spondias mombin L. shoot cultures were initiated from nodal explants taken from plants propagated by seeds. Explants coming from 4-6 months old plants, previously disinfected, were cultivated on WPM medium supplemented with a wide range of concentrations of BAP (0.0, 0.22, 0.44, 2.22 and 4.44 muM) and NAA (0.0, 0.27 and 2.70 muM). After four weeks, the responses obtained were axillary shoot and root formation. The first response were preferentially induced with the medium containing only BAP, regardless of the BAP concentration. The addition of NAA on medium reduced significantly axillary shoot formation and induced rhizogenesis. Roots were formed on nodal explant basis, preferentially on medium supplemented with 4.44 muM NAA. The medium supplemented with... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anacardiaceae; Hog plum tree; Axillary shoots. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452002000300054 |
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