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| Sá,Maria Eugênia Lisei de; Lopes,Marcus José Conceição; Campos,Magnólia de Araújo; Paiva,Luciano Vilela; Amorim,Regina Maria Santos de; Beneventi,Magda Aparecida; Firmino,Alexandre Augusto Pereira; Sá,Maria Fátima Grossi de. |
| Soybean is an important crop for Brazilian agribusiness. However, many factors can limit its production, especially root-knot nematode infection. Studies on the mechanisms employed by the resistant soybean genotypes to prevent infection by these nematodes are of great interest for breeders. For these reasons, the aim of this work is to characterize the transcriptome of soybean line PI 595099-Meloidogyne javanica interaction through expression analysis. Two cDNA libraries were obtained using a pool of RNA from PI 595099 uninfected and M. javanica (J2) infected roots, collected at 6, 12, 24, 48, 96, 144 and 192 h after inoculation. Around 800 ESTs (Expressed Sequence Tags) were sequenced and clustered into 195 clusters. In silico subtraction analysis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene expression; Root knot nematode; Transcriptome. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200008 |
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| Stolf-Moreira,Renata; Lemos,Eliana Gertrudes de Macedo; Abdelnoor,Ricardo Vilela; Beneventi,Magda Aparecida; Rolla,Amanda Alves Paiva; Pereira,Selma dos Santos; Oliveira,Maria Cristina Neves de; Nepomuceno,Alexandre Lima; Marcelino-Guimarães,Francismar Corrêa. |
| The objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: Gmβ-actin, GmGAPDH, GmLectin and GmRNAr18S. Total RNA was extracted from six samples: three from roots in a hydroponic system with different drought intensities (0, 25, 50, 75 and 100 minutes of water stress), and three from leaves of plants grown in sand with different soil moistures (15, 5 and 2.5% gravimetric humidity). The raw cycle threshold (Ct) data were analyzed, and the efficiency of each primer was calculated for an overall analysis of the Ct range among the different samples. The GeNorm application was used to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Expression stability; RT-qPCR; Water deficit. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000100008 |
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