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Extracting DNA of nematodes communities from Argentine Pampas agricultural soils Anais da ABC (AABC)
Mondino,Eduardo A.; Covacevich,Fernanda; Studdert,Guillermo A.; Pimentel,João P.; Berbara,Ricardo L.L..
We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agroecosystems; Nematodes; Nucleic acid extraction; CTAB.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200691
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Dominance of Paris-type morphology on mycothallus of Lunularia cruciata colonised by Glomus proliferum BJM
Fonseca,Henrique M.A.C.; Ferreira,Joana I.L.; Berbara,Ricardo L.L.; Zatorre,Natalia P..
Microscopic evidence confirms that L. cruciata hosting G. proliferum shows major anatomical traits (arbuscules, coils, arbusculate coils and vesicles) generally associated arbuscular mycorrhizal roots and the anatomical morphology of intra-thalli mycelium is predominantly of the Paris-type. Colonised L. cruciata showed a reduction of biomass when compared with axenic plants suggesting a drain of resources towards the fungus and depletion of nutrients required for optimum plant growth. The behaviour of mycothalli regarding available KH2PO4 indicates that the nutritional stress threshold for phosphorus (P) is above the residual amount of P already present in PhytagelTM and in plant inoculum. These raise the possibility that in certain circumstances the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arbuscular mycorrhizal fungi; Phosphorus; Arum-type; Liverwort; Monoxenic cultures.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100016
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