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| Registros recuperados: 11 | |
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| Camps,Rocío; Castro,Mónica; Besoain,Ximena. |
| R. Camps, M. Castro, and X. Besoain. 2014. Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCR. Cien. Inv. Agr. 41(2): 255-261. Citrus tristeza virus (CTV), Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd) are pathogens that affect citrus trees in Chile. The simultaneous use of a rapid diagnostic method with the effective cleaning of infected budwood is a powerful preventative method for controlling viral diseases. The objective of this study was the multiple detection of CTV, CEVd, and HSVd, through bio-amplification in Arizona 861-S1 citron plants, combined with two incubation temperatures and multiple RT-PCR in two steps. Citron indicator plants were inoculated with the three pathogens, both individually and... |
| Tipo: Journal article |
Palavras-chave: CEVd; Citrus; CTV; Diagnostic methods; HSVd; Rapid detection. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000200012 |
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| Polanco,Rubén; Pino,Constanza; Besoain,Ximena; Montealegre,Jaime; Pérez,Luz M. |
| The secretion of enzymes involved in biocontrol from the wild T. harzianum strains Th11 and Th12 and the mutants Th11A80.1, Th11C40.1 and Th12A10.1 was studied after their cultivation in the presence of R. solani cell walls as the sole carbon source. The results showed that endoprotease activity in the supernatants from Th11A80.1 and Th11C40.1 increased 2.77 and 4.79-fold, respectively, and the P-1,3-glucanase and P-1,4-chitinase activity from Th12A10.1 increased 1.86 and 1.54-fold, respectively, compared with the corresponding parental strains. The role of the three enzymes in the biocontrol of R. solani is discussed. |
| Tipo: Journal article |
Palavras-chave: SS-1; 3-glucanases endoproteases ß-1; 4-chitinases. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202015000200010 |
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| Montealegre,Jaime R; Ochoa,Fabián; Besoain,Ximena; Herrera,Rodrigo; Pérez,Luz M. |
| J.R. Montealegre, F. Ochoa, X. Besoain, R. Herrera, and L.M. Pérez. 2014. In vitro and glasshouse biocontrol of Rhizoctonia solani with improved strains of Trichoderma spp. Cien. Inv. Agr. 41(2):197-206. The potential of Trichoderma spp. fusants for the biocontrol of Rhizoctonia solani was compared with the ability of their corresponding parental strains. Their effect was tested in vitro using two R. solani strains, 509 (AG 2-1) and 618 (AG 4). The highest inhibitions in growth in dual cultures were obtained with the ThF2-1 (89.79%), ThF3-3 (90.55%), ThF4-15 (91.75%) and ThF5-8 (77.67%) fusants on R. solani 509; only ThF2-1 was able to inhibit the growth of R. solani 618 (60.19%). The inhibitory effect on growth was mainly due to diffusible metabolites.... |
| Tipo: Journal article |
Palavras-chave: Biological control; Methyl bromide; Tomato; Solanum lycopersicum. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000200006 |
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| Díaz,Gonzalo A; Auger,Jaime; Besoain,Ximena; Bordeu,Edmundo; Latorre,Bernardo A. |
| G. A., Diaz, J., Auger, X., Besoain, E., Bordeu, and B. A. Latorre, 2013. Prevalence and pathogenicity of fungi associated with grapevine trunk diseases in Chilean vineyards. Cien. Inv. Agr. 40(2): 327-339. Trunk diseases in grapevine (Vitis vinifera) have been identified as a major problem in the wine and table grape industries around the world, reducing the productivity, quality and longevity of vineyards. The present study examined 694 wood samples from the cordons and trunks of vines with trunk disease symptoms in 67 Chilean vineyards located between Copiapó (27°18' S) and Los Angeles (37°42' S). A total of 1,363 fungal isolates were obtained from diseased cordons and trunks with dark brown streaking, yellowish soft-spongy cankers and brown hard... |
| Tipo: Journal article |
Palavras-chave: Botryosphaeria canker; Esca; Fungal trunk pathogens; Vitis vinifera; Wood decay. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000200008 |
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| Besoain,Ximena; Bertolini,Edson; Camps,Rocío; Ramella,Fiona; Gorris,María Teresa; Torres,Margarita; Cambra,Mariano. |
| Citrus tristeza virus (CTV) was reported in the 1960's to affect Meyer lemon trees in Chile, but no field symptoms were observed. This study performed a complete biological, serological and molecular characterization of one hundred CTV isolates obtained from different hosts and geographical origins. Decline symptoms (DI) were not found on trees grafted onto sour orange. In Pica Oasis in northern Chile, stem pitting (SP) was found to affect grapefruit and Mexican lime trees. Most isolates present in the central area were considered mild (MCA-13-negative), while in the northern area aggressive isolates were observed and detected. Some of these isolates were capable of causing SP on grapefruit under field and greenhouse conditions and on sweet orange under... |
| Tipo: Journal article |
Palavras-chave: Biological indexing; CTV; Molecular characterization; Stem pitting; Seedling yellows. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202015000200011 |
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| Herrera,Rodrigo; Núñez,David; Romero,Natalia; Besoain,Ximena; Pérez,Luz María; Montealegre,Jaime. |
| The germination of conidia in wild-type (Th11, Th12 and Th650) and mutant (Th11A80.1, Th12A10.1 and Th650-NG7) strains of Trichoderma harzianum that were exposed to different commercial fungicides was studied. All wild-type and mutant Trichoderma strains were germinated in the presence of 1,700 mg L-1 of pencycuron. The wild-type strains Th12 and Th650 and the corresponding mutant strains Th12A10.1 and Th650-NG7 were sensitive to all concentrations of iprodione and metalaxil + mancozeb. The EC50 (Median Effective Concentration) values for the different fungicides were between 10-1 and 10-4 less than the concentrations recommended for field applications; one exception was Phyto-fos on Th650-NG7, where this ratio was 0.72. These results establish whether... |
| Tipo: Journal article |
Palavras-chave: Biocontrol; Biocontrol agents; Control of tomato root diseases; Fungicides; Fungicide tolerant Trichoderma; Integrated management. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300016 |
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| Montealegre,Jaime; Valderrama,Luis; Sánchez,Soledad; Herrera,Rodrigo; Besoain,Ximena; Pérez,Luz María. |
| Biocontrol of Rhizoctonia solani in tomatoes cultivated under greenhouse and field conditions was analyzed using the Trichoderma harzianum mutants Th650-NG7, Th11A80.1, Th12A40.1, Th12C40.1 and Th12A10.1 and ThF2-1, respectively. Their innocuousness on tomato cultivars 92.95 and Gondola (greenhouse assays), and on cultivar Fortaleza (field assays) was established. Alginate pellets (1.7 g pellets/L soil) containing c.a1 x 10(5) colony forming units (cfu)/g pellet were applied to a soil previously inoculated with R. solani at transplant (greenhouse) or to a naturally infected soil (field). Controls considered parental wild strains, a chemical fungicide and no additions. Th11A 80.1, Th12A10.1 and Th650-NG7 prevented the 100% mortality of tomato plants cv.... |
| Tipo: Journal article |
Palavras-chave: Bioantagonism; Field assays; Greenhouse assays; Root rot; Tomato. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200001 |
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| Montealegre,Jaime R.; Reyes,Rodrigo; Pérez,Luz María; Herrera,Rodrigo; Silva,Polyana; Besoain,Ximena. |
| Bacteria from the rhizoplane and surrounding soil of healthy and Rhizoctonia solani diseased tomato plants, cropped in greenhouse of the V Region of Chile, were collected. The best bacterial strains, based on their ability to control development of three R. solani isolates (identified as belonging to the anastomosis groups AG-2-1, AG‑4), were identified as B. subtilis (one isolate) and B. lentimorbus (two different isolates). All bacterial isolates resulted effective for the in vitro control of growth of all R. solani isolates, where the control mechanisms used by the bacteria do not involve the secretion of fungal cell wall hydrolytic enzymes. R. solani AG-2-1 was more sensitive than R. solani AG-4. On the other hand, all bacteria grew well in... |
| Tipo: Journal article |
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| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200006 |
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| Registros recuperados: 11 | |
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