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| Carnegie, Ryan; Meyer, Gary; Blackbourn, Janice; Cochennec, Nathalie; Berthe, Franck; Bower, Susan. |
| The protistan parasite Mikrocytos mackini, the causative agent of Denman Island disease in the oyster Crassostrea gigas in British Columbia, Canada, is of wide concern because it can infect other oyster species and because its life cycle, mode of transmission, and origins are unknown. PCR and fluorescent in situ hybridization (FISH) assays were developed for M mackini, the PCR assay was validated against standard histopathological diagnosis, and a preliminary phylogenetic analysis of the M mackini small-subunit ribosomal RNA gene (SSU rDNA) was undertaken. A PCR designed specifically not to amplify host DNA generated a 544 bp SSU rDNA fragment from M mackini-infected oysters and enriched M. mackini cell isolates, but not from uninfected control oysters.... |
| Tipo: Text |
Palavras-chave: Fluorescent in situ hybridization; PCR validation; Mikrocytos mackini; Denman Island disease. |
| Ano: 2003 |
URL: https://archimer.ifremer.fr/doc/2003/publication-2814.pdf |
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