|
|
| Pereira,Pedro Antonio Arraes; Oliver,Ann; Bliss,Fredrick Allen; Crowe,Lois; Crowe,John. |
| The objective of this study was to determine if trehalose can effectively enhance the viability and storage lifetime of Rhizobium tropici and Rhizobium etli after lyophilization as compared to the traditional protective combination of sucrose and peptone. Two strains of bean-nodulating rhizobia were effectively preserved in the lyophilized state by 100 mM trehalose, and survived for at least 12 days, even when stored under non-ideal conditions. Trehalose provided better protection than the sucrose/peptone combination. When protective excipients were introduced inside the cells, strain CFN 42 was more sensitive to the type of additive used than strain CIAT 899, probably because CIAT 899 produces beta (1-2) glucan, which may have a protective effect. beta... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Storage; Inoculation; Symbiosis; Glucan. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002000600012 |
| |
