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	Provedor de dados BABT (1) BJM (1) Autor Alfieri,Alice Fernandes (2) Alfieri,Amauri Alcindo (2) Bodnar,Lívia (2) Barry,Aline Fernandes (1) Bronkhorst,Dalton Evert (1) Fritzen,Juliana T.T. (1) Headley,Selwyn Arlington (1) Okano,Werner (1) Stipp,Danilo Tancler (1) Mais... Palavra-chave Bovine coronavirus (1) Brainstem dysfunctions (1) Cattle (1) Cell culture (1) Diarrhea (1) Epidemiology (1) Listeria monocytogenes (1) Listeriolysin gene (1) Molecular analyses (1) Neuropathology (1) Rhombencephalitis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2009 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Isolation in HRT-18 cells and molecular analysis of a BCoV strain from diarrheic feces of naturally infected calves BABT Stipp,Danilo Tancler; Barry,Aline Fernandes; Alfieri,Alice Fernandes; Bodnar,Lívia; Alfieri,Amauri Alcindo. Bovine coronavirus (BCoV) may cause acute diarrhea in newborn calves, leading to significant economic losses for cattle farmers. There are several diagnostic techniques used to detect BCoV in calf fecal samples, but virus isolation still has advantages for antigenic and genomic characterization. This study describes the isolation in HRT-18 cells and molecular characterization of Brazilian BCoV wild-type strains. Three fecal samples from diarrheic 30 day-old calves were inoculated in HRT-18 cell monolayers, which were then evaluated for HA titers and tested using semi-nested PCR followed by RFLP and sequencing. Two samples were successfully isolated and presented HA titers of 16 and 32 units per 25 mL. The results were confirmed using semi-nested PCR and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Diarrhea; Bovine coronavirus; Cell culture; Molecular analyses. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700007 Histopathological and molecular characterization of encephalitic listeriosis in small ruminants from northern Paraná, Brazil BJM Headley,Selwyn Arlington; Bodnar,Lívia; Fritzen,Juliana T.T.; Bronkhorst,Dalton Evert; Alfieri,Alice Fernandes; Okano,Werner; Alfieri,Amauri Alcindo. Listeriosis is a disease primarily of ruminants caused by the Gram-positive bacterium Listeria monocytogenes. Ruminants either demonstrate manifestations of the encephalitic, septicemic, or reproductive form of listeriosis. The pathological and molecular findings with encephalitic listeriosis in a 5.5-month-old, male, mixed-breed goat and a 3-year-old Texel-crossed sheep from northern Paraná, Brazil are described. Clinically, the kid demonstrated circling, lateral protrusion of the tongue, head tilt, and convulsions; the ewe presented ataxia, motor incoordination, and lateral decumbency. Brainstem dysfunctions were diagnosed clinically and listeriosis was suspected. Necropsy performed on both animals did not reveal remarkable gross lesions; significant... Tipo: Info:eu-repo/semantics/article Palavras-chave: Brainstem dysfunctions; Rhombencephalitis; Neuropathology; Listeriolysin gene; Epidemiology; Listeria monocytogenes. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300036 Registros recuperados: 2 Primeira ... 1 ... Última

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