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	Provedor de dados BJID (2) Autor Boettger,Bruno Cruz (2) Cappellano,Paola (2) Pignatari,Antonio Carlos Campos (2) Quiles,Milene Gonçalves (2) Carlesse,Fabianne (1) Carvalhaes,Cecilia Godoy (1) Inoue,Fernanda Matsiko (1) Monteiro,Jussimara (1) Ponzio,Vinicius (1) Santos,Daniel Wagner (1) Souza,Ingvar Ludwig Augusto de (1) Mais... Palavra-chave Bloodstream infections (1) Communication (1) Febrile Neutropenia (1) Mass Spectrometry (1) Mass spectrometry (1) Molecular Diagnosis (1) Molecular diagnosis (1) Molecular panels (1) PCR (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/report (1) Ano 2019 (1) 2018 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry and real-time PCR in a combined protocol for diagnosis of bloodstream infections: a turnaround time approach BJID Quiles,Milene Gonçalves; Boettger,Bruno Cruz; Inoue,Fernanda Matsiko; Monteiro,Jussimara; Santos,Daniel Wagner; Ponzio,Vinicius; Carlesse,Fabianne; Cappellano,Paola; Carvalhaes,Cecilia Godoy; Pignatari,Antonio Carlos Campos. ABSTRACT Bloodstream infections (BSIs) are serious infections associated with high rates of morbidity and mortality. Every hour delay in initiation of an effective antibiotic increases mortality due to sepsis by 7%. Turnaround time (TAT) for conventional blood cultures takes 48 h, forcing physicians to streamline therapy by exposing patients to broad-spectrum antimicrobials. Our objective was (1) to evaluate the accuracy and TAT of an optimized workflow combining direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and in-house real-time polymerase chain reaction (PCR) for bacterial identification and antimicrobial resistance profiling directly from positive blood bottles for diagnosing bloodstream infections... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bloodstream infections; Molecular diagnosis; Molecular panels; PCR; Mass spectrometry. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300164 Tailoring antimicrobials in febrile neutropenia: using faster diagnostic and communication tools to improve treatment in the era of extensively resistant pathogens BJID Souza,Ingvar Ludwig Augusto de; Quiles,Milene Gonçalves; Boettger,Bruno Cruz; Pignatari,Antonio Carlos Campos; Cappellano,Paola. ABSTRACT Febrile Neutropenia represents a medical emergency and the use of appropriate antimicrobial therapy is essential for a better outcome. Although being time-consuming, conventional cultures and antimicrobial susceptibility tests remain the golden standard practices for microbiology identification. Final reports are typically available within several days. Faster diagnostic tools, such as species identification trough Matrix Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) and molecular techniques might help to shorten time to diagnostic and also guide definitive therapy in this scenario. Here we present a case in which the use of a diagnostic molecular workflow combining MALDI-TOF and real-time PCR for relevant genes codifying... Tipo: Info:eu-repo/semantics/report Palavras-chave: Febrile Neutropenia; Molecular Diagnosis; Mass Spectrometry; Communication. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000300239 Registros recuperados: 2 Primeira ... 1 ... Última

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