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Amiraux, Rémi; Burot, Christopher; Bonin, Patricia; Massé, Guillaume; Guasco, Sophie; Babin, Marcel; Vaultier, Frédéric; Rontani, Jean-françois. |
During sea-ice melt in the Arctic, primary production by sympagic (sea-ice) algae can be exported efficiently to the seabed if sinking rates are rapid and activities of associated heterotrophic bacteria are limited. Salinity stress due to melting ice has been suggested to account for such low bacterial activity. We further tested this hypothesis by analyzing samples of sea ice and sinking particles collected from May 18 to June 29, 2016, in western Baffin Bay as part of the Green Edge project. We applied a method not previously used in polar regions—quantitative PCR coupled to the propidium monoazide DNA-binding method—to evaluate the viability of bacteria associated with sympagic and sinking algae. We also measured cis-trans isomerase activity, known to... |
Tipo: Text |
Palavras-chave: Bacteria; Sea ice; Sympagic algae; Ice biota; Viability; Stress factors; Salinity; Bactericidal free fatty acids; Carbon export. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00681/79329/81840.pdf |
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Garel, Marc; Bonin, Patricia; Martini, Severine; Guasco, Sophie; Roumagnac, Marie; Bhairy, Nagib; Armougom, Fabrice; Tamburini, Christian. |
The pelagic realm of the dark ocean is characterized by high hydrostatic pressure, low temperature, high-inorganic nutrients, and low organic carbon concentrations. Measurements of metabolic activities of bathypelagic bacteria are often underestimated due to the technological limitations in recovering samples and maintaining them under in situ environmental conditions. Moreover, most of the pressure-retaining samplers, developed by a number of different labs, able to maintain seawater samples at in situ pressure during recovery have remained at the prototype stage, and therefore not available to the scientific community. In this paper, we will describe a ready-to-use pressure-retaining sampler, which can be adapted to use on a CTD-carousel sampler. As well... |
Tipo: Text |
Palavras-chave: Pressure-retaining sampler; Deep sea; In situ sampling; Prokaryotic activities; Prokaryotic diversity. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00688/79992/82967.pdf |
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