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One-step, codominant detection of imidazolinone resistance mutations in weedy rice (Oryza sativa L.) 69
Rosas,Juan E; Bonnecarrère,Victoria; Pérez de Vida,Fernando.
Background Weedy rice (Oryza sativa L.) is a noxious form of cultivated rice (O. sativa L.) associated with intensive rice production and dry seeding. A cost-efficient strategy to control this weed is the Clearfield rice production system, which combines imidazolinone herbicides with mutant imidazolinone-resistant rice varieties. However, imidazolinone resistance mutations can be introgressed in weedy rice populations by natural outcrossing, reducing the life span of the Clearfield technology. Timely and accurate detection of imidazolinone resistance mutations in weedy rice may contribute to avoiding the multiplication and dispersion of resistant weeds and to protect the Clearfield system. Thus, highly sensitive and specific methods with high throughput...
Tipo: Journal article Palavras-chave: Clearfield rice; DNA-based resistance diagnosis; Herbicide resistance; KASP; Red rice; SNP.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000200007
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Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi 69
Lourdes Rocha,Carla Maria; Vellicce,Gabriel Ricardo; García,María Gabriela; Pardo,Esteban Mariano; Racedo,Josefina; Perera,María Francisca; de Lucía,Adrian; Gilli,Javier; Bogado,Noelia; Bonnecarrère,Victoria; German,Silvia; Marcelino,Francismar; Ledesma,Fernando; Reznikov,Sebastián; Ploper,Leonardo Daniel; Welin,Bjorn; Castagnaro,Atilio Pedro.
Background Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of...
Tipo: Journal article Palavras-chave: Asian soybean rust; Genetic variation; Glycine max; Molecular markers.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600009
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