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| Pino-Lagos,Karina; Michea,Paula; Sauma,Daniela; Alba,Andrea; Morales,Jorge; Bono,María Rosa; Fierro,Alberto; Rosemblatt,Mario. |
| One of the mechanisms for generation of tolerance involves immature dendritic cells (DCs) and a subpopulation of regulatory CD4+ CD25+ T lymphocytes (T REG). The purpose of this work was to analyze how Cyclosporine A (CsA), a widely used immunosuppressive drug, may affect T REG proliferation. Purified and activated murine DCs obtained from bone marrow precursors differentiated with rGMCSF were co-cultured with purified CFSE-labeled T REG from OTII mice, and their phenotype and proliferation analyzed by flow cytometry. Our data indicate that DCs differentiated in the presence of CsA show an altered phenotype, with a lower expression of MHC-II and a lower activating capacity. Additionally, these CsA-treated DCs show decreased production of IL-2 and IL-12 and... |
| Tipo: Journal article |
Palavras-chave: CD4+CD25+ regulatory T cell; Cyclosporine A; Dendritic cells; Transplant tolerance. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300010 |
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