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The use of vinasse as an amendment to ex-situ bioremediation of soil and groundwater contaminated with diesel oil BABT
Mariano,Adriano Pinto; Crivelaro,Sérgio Henrique Rezende; Angelis,Dejanira de Franceschi de; Bonotto,Daniel Marcos.
This work investigated the possibility of using vinasse as an amendment in ex-situ bioremediation processes. Groundwater and soil samples were collected at petrol stations. The soil bioremediation was simulated in Bartha biometer flasks, used to measure the microbial CO2 production, during 48 days, where vinasse was added at a concentration of 33 mL.Kg-1of soil. Biodegradation efficiency was also measured by quantifying the total petroleum hydrocarbons (TPH) by gas chromatography. The groundwater bioremediation was carried out in laboratory experiments simulating aerated (bioreactors) and not aerated (BOD flasks) conditions. In both the cases, the concentration of vinasse was 5 % (v/v) and different physicochemical parameters were evaluated during 20 days....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioremediation; Soil; Groundwater; Diesel oil; Biostimulation; Vinasse.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400030
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Investigation about the efficiency of the bioaugmentation technique when applied to diesel oil contaminated soils BABT
Mariano,Adriano Pinto; Angelis,Dejanira de Franceschi de; Pirôllo,Maria Paula Santos; Contiero,Jonas; Bonotto,Daniel Marcos.
This work investigated the efficiency of the bioaugmentation technique when applied to diesel oil contaminated soils collected at three service stations. Batch biodegradation experiments were carried out in Bartha biometer flasks (250 mL) used to measure the microbial CO2 production. Biodegradation efficiency was also measured by quantifying the concentration of hydrocarbons. In addition to the biodegradation experiments, the capability of the studied cultures and the native microorganisms to biodegrade the diesel oil purchased from a local service station, was verified using a technique based on the redox indicator 2,6 -dichlorophenol indophenol (DCPIP). Results obtained with this test showed that the inocula used in the biodegradation experiments were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioremediation; Bioaugmentation; Soil; Diesel oil; Service station.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500030
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Biodegradability of commercial and weathered diesel oils BJM
Mariano,Adriano Pinto; Bonotto,Daniel Marcos; Angelis,Dejanira de Franceschi de; Pirôllo,Maria Paula Santos; Contiero,Jonas.
This work aimed to evaluate the capability of different microorganisms to degrade commercial diesel oil in comparison to a weathered diesel oil collected from the groundwater at a petrol station. Two microbiological methods were used for the biodegradability assessment: the technique based on the redox indicator 2,6 - dichlorophenol indophenol (DCPIP) and soil respirometric experiments using biometer flasks. In the former we tested the bacterial cultures Staphylococcus hominis, Kocuria palustris, Pseudomonas aeruginosa LBI, Ochrobactrum anthropi and Bacillus cereus, a commercial inoculum, consortia obtained from soil and groundwater contaminated with hydrocarbons and a consortium from an uncontaminated area. In the respirometric experiments it was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradability; Bioremediation; Commercial oil diesel; Weathered diesel oil.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100028
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Laboratory study on the bioremediation of diesel oil contaminated soil from a petrol station BJM
Mariano,Adriano Pinto; Kataoka,Ana Paula de Arruda Geraldes; Angelis,Dejanira de Franceschi de; Bonotto,Daniel Marcos.
The purpose of the present study was to investigate possible methods to enhance the rate of aerobic biodegradation of hydrocarbons (ex-situ treatments). In this work, the bioremediation processes were applied to a sandy soil with a high level of contamination originated from the leakage of a diesel oil underground storage tank at a petrol station. Laboratory scale experiments (Bartha biometer flasks) were used to evaluate the biodegradation of the diesel oil. Enhancement of biodegradation was carried out through biostimulation (addition of nitrogen and phosphorus solutions or Tween 80 surfactant) and bioaugmentation (bacterial consortium isolated from a landfarming system). To investigate interactions between optimizing factors, and to find the right...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioremediation; Soil; Diesel Oil; Petrol Station.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200030
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