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| Payan, P; Borelli, G; Priouzeau, F; De Pontual, Helene; Boeuf, G; Mayer Gostan, N. |
| Kinetic and pharmacological characteristics of Ca2+ fluxes across the saccular epithelium of trout were studied using a perfused isolated inner ear. Ca-45(2+) influx from the Ringer solution to the endolymph was 3-4 nmoles h(-1) mu(-1) endolymph, which corresponds to a global turnover rate of the endolymph calcium of 200% h(-1). Ca2+ entry into the proximal endolymph was faster than into the distal fluid. Net Ca2+ movement across the saccular epithelium depended on the direction and intensity of the chemical gradient of calcium between the Ringer solution and the endolymph. Increasing the calcium concentration in the Ringer solution up to 4.4 mmol l(-1) provoked an accumulation of Ca2+ in both proximal and distal endolymphs, and equilibrium was reached... |
| Tipo: Text |
Palavras-chave: Inner ear; Perfusion; Otolith; Endolymph; Flux; Calcium; Oncorhynchus mykiss; Trout. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-416.pdf |
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| Borelli, G; Guibbohni, M; Mayer Gostan, N; Priouzeau, France; De Pontual, Helene; Allemand, D; Puverel, S; Tambutte, E; Payan, P. |
| Ionic and organic parameters of the otolith calcification process in the trout Oncorhynchus mykiss were analysed in plasma and endolymph over the day:night cycle. Plasma pH remained constant and total CO2 concentration was significantly lower (by 21%) during the day than at night. Calcifying parameters (total CO2, total calcium concentration) were measured in the proximal and distal endolymphs and were unchanged in the latter during the day:night cycle, but fluctuated in the former. Non-collagenous protein and collagen concentrations in endolymph were higher (1.5- and 10-fold, respectively) during the day than at night. As there was no change in total calcium concentration, we propose that Ca2+ increases during the dark period and was maximal by the end of... |
| Tipo: Text |
Palavras-chave: Endolymph; Day night cycle; Calcification; Otolith. |
| Ano: 2003 |
URL: https://archimer.ifremer.fr/doc/2003/publication-418.pdf |
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