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| Mendonça,A.C.; Carneiro,E.M.; Bosqueiro,J.R.; Crepaldi-Alves,S.C.; Boschero,A.C.. |
| We studied the development of the insulin secretion mechanism in the pancreas of fetal (19- and 21-day-old), neonatal (3-day-old), and adult (90-day-old) rats in response to stimulation with 8.3 or 16.7 mM glucose, 30 mM K+, 5 mM theophylline (Theo) and 200 µM carbamylcholine (Cch). No effect of glucose or high K+ was observed on the pancreas from 19-day-old fetuses, whereas Theo and Cch significantly increased insulin secretion at this age (82 and 127% above basal levels, respectively). High K+ also failed to alter the insulin secretion in the pancreas from 21-day-old fetuses, whereas 8.3 mM and 16.7 mM glucose significantly stimulated insulin release by 41 and 54% above basal levels, respectively. Similar results were obtained with Theo and Cch. A more... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rat islet cell; Glucose; Pancreatic islets; Carbamylcholine; Theophylline; Potassium; Insulin secretion. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600018 |
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| Rafacho,A.; Giozzet,V.A.G.; Boschero,A.C.; Abrantes,J.L.F.; Cestari,T.M.; Carneiro,E.M.; Bosqueiro,J.R.. |
| A low-protein diet leads to functional and structural pancreatic islet alterations, including islet hypotrophy. Insulin-signaling pathways are involved in several adaptive responses by pancreatic islets. We determined the levels of some insulin-signaling proteins related to pancreatic islet function and growth in malnourished rats. Adult male Wistar rats (N = 20 per group) were fed a 17% protein (normal-protein diet; NP) or 6% protein (low-protein diet; LP), for 8 weeks. At the end of this period, blood glucose and serum insulin and albumin levels were measured. The morphometric parameters of the endocrine pancreas and the content of some proteins in islet lysates were determined. The β-cell mass was significantly reduced (≅65%) in normoglycemic but... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Β-cell mass; Insulin signaling; Low-protein diet; Pancreatic islets; Protein phosphorylation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000010 |
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