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| Registros recuperados: 273 | |
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| Bouilly, Karine; Leitao, Alexandra; Mccombie, Helen; Chaves, R.; Guedes Pinto, H; Boudry, Pierre; Lapegue, Sylvie. |
| In the Pacific oyster, Crassostrea gigas hypodiploid aneuploid cells with 2n=19, 18 or 17 chromosomes have regularly been reported. There is a negative correlation between somatic aneuploidy and growth rate in oysters (Leitão et al., 2001a) as well as evidence for a genetic basis (Leitão et al., 2001b). Furthermore, non-random chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique (Leitão et al., 2001c). This poster summarises different studies used to investigate the effects of the herbicide atrazine on the level of aneuploidy in this species |
| Tipo: Text |
Palavras-chave: Genetic; Aneuploidy; Toxicology; Impact; Atrazine; Crassostrea gigas; Pacific oysters. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/acte-3489.pdf |
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| Petton, Bruno; Boudry, Pierre; Alunno-bruscia, Marianne; Pernet, Fabrice. |
| Mortalities of oyster Crassostrea gigas seed associated with ostreid herpesvirus OsHV-1 μVar have been observed in many oyster-producing countries since 2008. The present study, comprised of 4 complementary experiments, aimed to identify factors associated with disease-induced oyster mortality in order to propose mitigation strategies. Our first experiment compared survival of oysters from natural spatfall with others sampled from nurseries, after thermal elevation in the laboratory from <14 to 21°C. A total of 60% of the tested wild seed batches (n = 51) were infected by OsHV-1, exhibited mortality and were able to transmit the disease to cohabited naïve oysters. Comparatively, only 1 out of the 32 tested batches sampled from nurseries presented... |
| Tipo: Text |
Palavras-chave: Epidemiology; Risk analyses; Shellfish farming; Virus OsHV-1 mu Var. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00257/36777/35390.pdf |
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| Foulon, Valentin; Artigaud, Sebastien; Buscaglia, Manon; Bernay, Benoit; Fabioux, Caroline; Petton, Bruno; Elies, Philippe; Boukerma, Kada; Hellio, Claire; Guerard, Fabienne; Boudry, Pierre. |
| Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagic larvae settle prior to metamorphosis to a benthic stage. The oyster Crassostrea gigas has been extensively studied for its economic and ecological importance. However, the bioadhesive produced by ready to settle larvae of this species has been little studied. The pediveliger stage of oysters is characterized by the genesis of a specific organ essential for adhesion, the foot. Our scanning electron microscopy and histology analysis revealed that in C. gigas the... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00462/57379/59452.pdf |
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| Degremont, Lionel; Bedier, Edouard; Boudry, Pierre. |
| Response to divergent selection for "high" and "low" survival during the summer period, from July to October, was investigated in juvenile (six-month-old) Pacific oysters, Crassostrea gigas, by producing two sets of progenies in 2002 (Generation 2) and three sets of progenies in 2003 (Generation 3). A strict between-family approach was used and resistance of these selected progenies to summer mortality was assessed in three sites along French coasts, to determine their response to selection and estimate realized heritability of the trait. A significant difference in survival was observed between the "high" and "low" selected groups at all sites for all sets of progenies, indicating a significant genetic component. High realized heritabilities for survival... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Heritability; Juveniles; Summer mortality; Survival; Growth. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6950.pdf |
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| Sussarellu, Rossana; Huvet, Arnaud; Lapegue, Sylvie; Quillen, Virgile; Lelong, Christophe; Cornette, Florence; Jensen, Lasse Fast; Bierne, Nicolas; Boudry, Pierre. |
| Background Originating from Northeast Asia, the Pacific oyster Crassostrea gigas has been introduced into a large number of countries for aquaculture purpose. Following introduction, the Pacific oyster has turned into an invasive species in an increasing number of coastal areas, notably recently in Northern Europe. Methods To explore potential adaptation of reproductive traits in populations with different histories, we set up a common garden experiment based on the comparison of progenies from two populations of Pacific oyster sampled in France and Denmark and their hybrids. Sex ratio, condition index and microarray gene expression in gonads, were analyzed in each progeny (n = 60). Results A female-biased sex-ratio and a higher condition index were... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Adaptation; Invasiveness; Fertility; Sex-ratio; Transcriptome; Microarray. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00287/39829/38343.pdf |
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| Soletchnik, Patrick; Huvet, Arnaud; Le Moine, Olivier; Razet, Daniel; Geairon, Philippe; Faury, Nicole; Goulletquer, Philippe; Boudry, Pierre. |
| This paper reports a comparative study of two cupped oysters, Crassostrea gigas and C angulata, and their hybrids reared in the Marennes-Oleron Sound (France) under usual farming conditions. The relative proficiency of these species was characterized in terms of growth, survival and reproduction, three major concerns for oyster farming. Four populations of each taxon were sampled (C gigas: Japan and France; C angulata: Taiwan and Spain) and used as progenitors to produce six different progenies under common hatchery and nursery conditions (transfer of 5 to 6 g of seed oysters at sea and rearing in bags for 2 years). Mortality profiles differed greatly among the progenies. The C angulata progeny of Taiwanese origin gave the poorest results, with 100%... |
| Tipo: Text |
Palavras-chave: Atlantic coast; Crassostrea gigas; Phenotypes; Hybrids; Oyster fanning. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-560.pdf |
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| Boudry, Pierre; Collet, B; Mccombie, Helen; Ernande, Bruno; Morand, B; Heurtebise, Serge; Gerard, Andre. |
| In order to study individual growth variability and its relationship with survival in juvenile Crassostrea gigas, parental oysters were sampled at four sites located along the French Atlantic coast and bred under controlled hatchery conditions. Four groups of larvae were obtained by crossing five males and five females from each of the four sites, and a fifth group by crossing these 20 males and 20 females together in a pool. Larvae were reared under conditions allowing the maintenance of a maximum variability of size and gave five experimental groups. Oysters were individually monitored for growth and survival from 3 to 10 months after fertilization. The individual growth performances were relatively stable over time and no noticeable compensation for... |
| Tipo: Text |
Palavras-chave: Survival; Oysters; Growth; Crassostrea gigas; Aquaculture. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-467.pdf |
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| Boudry, Pierre; Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie; Heurtebise, Serge; Ledu, Christophe; Blouin, Frederic; Phelipot, Pascal; Gerard, Andre. |
| L'étude de la variabilité génétique de l' huître creuse Crassastrea gigas est un point essentiel dans la mise en place de programmes de gestion des ressources génétiques et de sélection chez cette espèce. C. gigas, originaire du Japon, a été introduite dans de nombreuses régions du monde et représente aujourd'hui plus de 95 % de la production mondiale d' huîtres. Introduite en France au début des années 1970 pour remplacer l' huître portugaise Crassostrea angulata, c'est aujourd 'hui une ressource de première importance pour notre région. Malgré la maîtrise du cycle de reproduction de l'espèce et son importance commerciale, elle n' a pas été génétiquement améliorée et on peut donc considérer les stocks comme « sauvages» ou « non domestiqués ». De plus, la... |
| Tipo: Text |
Palavras-chave: Génétique; Ressources génétiques; Différenciation génétique; Hybridation; Anomalies chromosomiques; Huîtres creuses; Crassostrea gigas; Crassostrea angulata. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00044/15564/12955.pdf |
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| Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumon, Andy; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie. |
| Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in its aquaculture production. Selective breeding programs for resistance to bonamiosis have been undertaken to counter this disease. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters injected with the parasite to two tested families of oysters (20 and 8-month old at the beginning of the experiment, with different genetic backgrounds) originating from a selective breeding program developed by IFREMER in France. Mortalities were checked daily and ventricular heart smears were performed on dying or moribund oysters to detect the... |
| Tipo: Text |
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4783.pdf |
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| Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie. |
| Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci... |
| Tipo: Text |
Palavras-chave: Invasive; AFLP; Genome scan; Crassotrea gigas; Pacific oyster. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4805.pdf |
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| Lallias, Delphine; Gomez-raya, L.; Haley, C. S.; Arzul, Isabelle; Heurtebise, Serge; Beaumont, A. R.; Boudry, Pierre; Lapegue, Sylvie. |
| We have identified quantitative trait loci (QTL) in the flat oyster (Ostrea edulis) for resistance to Bonamia ostreae, a parasite responsible for the dramatic reduction in the aquaculture of this species. An F-2 family from a cross between a wild oyster and an individual from a family selected for resistance to bonamiosis was cultured with wild oysters injected with the parasite, leading to 20% cumulative mortality. Selective genotyping of 92 out of a total of 550 F-2 progeny (i.e., 46 heavily infected oysters that died and 46 parasite-free oysters that survived) was performed using 20 microsatellites and 34 amplification fragment length polymorphism primer pairs. Both a two-stage testing strategy and QTL interval mapping methods were used. The two-stage... |
| Tipo: Text |
Palavras-chave: Statistical power; Survival analysis; QTL mapping; Disease resistance; Ostrea edulis. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6682.pdf |
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| Degremont, Lionel; Boudry, Pierre; Ropert, Michel; Samain, Jean-francois; Bedier, Edouard; Soletchnik, Patrick. |
| This study investigated the effects of age (first or second summer of life) and environment (three study sites on French coasts) on summer mortality in Crassostrea gigas, using three generations of oysters produced during the Morest project. For each generation, two groups of oysters, selected either as 'resistant' (R) or 'susceptible' (S) to summer mortality at the age of 6 months in Rivière d'Auray (RA, South Brittany), were evaluated. In Ronce (Marennes-Oléron Bay) and RA, R oysters had low mortality during their first and second summers, while mortality was higher during the second summer in Baie des Veys (BDV, Normandy). In RA, S oysters exhibited higher mortality during their first summer than during their second, while the opposite result was found... |
| Tipo: Text |
Palavras-chave: Summer mortality; Age; Environment; Genetic; Crassostrea gigas. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6972.pdf |
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| Genard, Bertrand; Miner, Philippe; Nicolas, Jean-louis; Moraga, Dario; Boudry, Pierre; Pernet, Fabrice; Tremblay, Rejean. |
| Background: Bacterial infections are common in bivalve larvae and can lead to significant mortality, notably in hatcheries. Numerous studies have identified the pathogenic bacteria involved in such mortalities, but physiological changes associated with pathogen exposure at larval stage are still poorly understood. In the present study, we used an integrative approach including physiological, enzymatic, biochemical, and molecular analyses to investigate changes in energy metabolism, lipid remodelling, cellular stress, and immune status of Crassostrea gigas larvae subjected to experimental infection with the pathogenic bacteria Vibrio coralliilyticus. Findings: Our results showed that V. coralliilyticus exposure induced (1) limited but significant increase... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00144/25504/23662.pdf |
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| Cochennec-laureau, Nathalie; Baud, Jean-pierre; Pepin, Jean-francois; Benabdelmouna, Abdellah; Soletchnik, Patrick; Lupo, Coralie; Garcia, Celine; Arzul, Isabelle; Boudry, Pierre; Huvet, Arnaud; Pernet, Fabrice; Bachere, Evelyne; Bedier, Edouard; Petton, Bruno; Gaussem, Florian; Stanisiere, Jean-yves; Degremont, Lionel. |
| Depuis 2008, la filière ostréicole française doit faire face à des surmortalités exceptionnelles des naissains d’huîtres creuses, Crassostrea gigas, (huîtres de moins d’un an) comprises entre 60 et 90% dans tous les sites d’élevage. Des cas de mortalité similaires ont été décrits en Irlande, dans les îles anglo-normandes, au Portugal en 2009 et au Royaume-Uni en 2010. Récemment deux épisodes de mortalité, associés à la présence d’un virus OsHV1 μvar (ou un variant très proche) ont été également décrits en Nouvelle Zélande (décembre 2010) et en Australie (janvier 2011) soulignant la vulnérabilité de la filière ostréicole dans le monde. L’objectif de ce document est de faire un point sur les recherches menées depuis l’apparition de ce phénomène et de... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00033/14423/11719.pdf |
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| Morvezen, Romain; Cornette, Florence; Charrier, Gregory; Guinand, Bruno; Lapegue, Sylvie; Boudry, Pierre; Laroche, Jean. |
| We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops. |
| Tipo: Text |
Palavras-chave: Microsatellites; Polymerase chain reaction; Parentage; Assignment; Bivalve mollusc. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00137/24822/22907.pdf |
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| Lallias, Delphine; Beaumont, Andy; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Lapegue, Sylvie. |
| A breeding programme has been undertaken by Ifremer to produce selected Ostrea edulis families which are resistant to Bonamia ostreae, a parasite which is the most significant constraint on aquaculture of this oyster. Using such families, we aimed to identify Quantitative Trait Loci (QTLs) of resistance/susceptibility to bonamiosis. We used an F2 family derived from a bi-parental cross between a wild oyster and an individual from one of the selected oyster families. A total of 550 F2 individuals from the family cohabited with wild oysters that were injected with the parasite. Mortality was checked daily over six months and heart smears were used on moribund or dying oysters to detect the presence and level of infection of the parasite. Ninety two F2... |
| Tipo: Text |
Palavras-chave: QTLs; Flat oyster; Ostrea edulis; Bonamia ostreae; Genetic; QTL. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-3726.pdf |
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| Lapegue, Sylvie; Taris, Nicolas; Lallias, Delphine; Bonhomme, Francois; Boudry, Pierre. |
| The European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. The latest results obtained on the genetic differentiation between these populations have led us to persue studies at a finer scale, in order to estimate the effective number of breeders and the temporal dynamics of reproduction and, more specially, recruitment. Several experiments were performed to document (1) the variance in allele frequencies during a natural settlement period, (2) the paternal contribution to fertilization by analyzing larvae sampled at the brooding stage within individual females, (3) the variance of individual... |
| Tipo: Text |
Palavras-chave: Recruitment; Microsatellite markers; Ostrea edulis; European flat oyster; Genetic. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/acte-3485.pdf |
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| Lallias, Delphine; Boudry, Pierre; Batista, Frederico; Beaumont, Andy; King, Jonathan W.; Turner, John R.; Lapegue, Sylvie. |
| The Pacific oyster, Crassostrea gigas, native to northeast Asia, is one of the most important cultured shellfish species. In Europe, Pacific oysters first settled along the Atlantic coasts of France at the end of the 1960s but rapidly spread and are now widely established. Twenty-two sites in the United Kingdom (UK), Ireland, Denmark, France and Spain were sampled to assess genetic diversity and differentiation. Hatchery-propagated stocks from two hatcheries located in the UK also were included. Two main genetic clusters were identified from pairwise genetic differentiation indexes, Bayesian clustering methods or neighbour-joining analysis, based on 7 microsatellite loci: (1) a Northeast cluster (including feral samples from East England, Ireland and... |
| Tipo: Text |
Palavras-chave: Invasion genetics; Marine mollusc; Microsatellites; MtDNA; Pacific oyster; Crassostrea gigas. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00268/37973/36052.pdf |
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| Meistertzheim, Anne-leila; Arnaud-haond, Sophie; Boudry, Pierre; Thebault, Marie-therese. |
| As a result of aquaculture activities, Pacific oysters Crassostrea gigas (Thunberg, 1793) have invaded European coasts. Using seven microsatellites, we found virtually no genetic differentiation between natural populations throughout the European range (from the south of the Wadden Sea (the Netherlands) to the south of France) and French cultivated oysters. The genetic homogeneity of Pacific oyster samples appears to be the result of repeated transfers from same seed stocks made for aquaculture and, to a lesser extent, widespread dispersal due to specific biological traits of this species. The only genetic differentiation of Sylt population in the north of the Wadden Sea (Germany) suggests a stronger, persistent impact of ongoing supply of new genetic... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00119/23051/20924.pdf |
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| Registros recuperados: 273 | |
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