|
|
|
| Registros recuperados: 35 | |
|
|
| Fleury, Elodie; Huvet, Arnaud; Lelong, Christophe; De Lorgeril, Julien; Boulo, Viviane; Gueguen, Yannick; Bachere, Evelyne; Tanguy, Arnaud; Moraga, Dario; Fabioux, Caroline; Lindeque, Penelope; Shaw, Jenny; Reinhardt, Richard; Prunet, Patrick; Davey, Grace; Lapegue, Sylvie; Sauvage, Christopher; Corporeau, Charlotte; Moal, Jeanne; Gavory, Frederick; Wincker, Patrick; Moreews, Francois; Klopp, Christophe; Mathieu, Michel; Boudry, Pierre; Favrel, Pascal. |
| Background: Although bivalves are among the most-studied marine organisms because of their ecological role and economic importance, very little information is available on the genome sequences of oyster species. This report documents three large-scale cDNA sequencing projects for the Pacific oyster Crassostrea gigas initiated to provide a large number of expressed sequence tags that were subsequently compiled in a publicly accessible database. This resource allowed for the identification of a large number of transcripts and provides valuable information for ongoing investigations of tissue-specific and stimulus-dependant gene expression patterns. These data are crucial for constructing comprehensive DNA microarrays, identifying single nucleotide... |
| Tipo: Text |
|
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6770.pdf |
| |
|
|
| Robledo, Jaf; Boulo, Viviane; Mialhe, Eric; Despres, Béatrice; Figueras, Antonio. |
| Digestive glands of mussels Mytilus edulis from Brittany, France, infected with Marteilia sp. (Ascetospora) were used to purify the parasite. A modification of a previously used purification protocol increased purification efficiency, permitting sporangial primordia and sporangia of Marteilia sp. to be obtained. Mouse (Balb/c) monoclonal antibodies were generated against this parasite. From the fusion, 26 monoclonal antibodies against Marteilia sp. were obtained. Antibodies from 6 clones reacted only with Marteilia sp. cells and not with normal host tissues. Four of these antibodies (1/1-3, 3/1-1, 4/1-1 and 6/2-3) reacted with the sporangia wall and two with the spore cytoplasm (9/1-1 and 12/5-1). Antibodies cross-reacted with Marteilia refringens from... |
| Tipo: Text |
Palavras-chave: Marteilia sp.; Mytilus edulis; Monoclonal antibodies. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/00196/30730/29404.pdf |
| |
|
| |
|
|
| Boulo, Viviane; Hervio, Dominique; Morvan, Annie; Bachere, Evelyne; Mialhe, Eric. |
| The chief importance of hemocytes as immune effectors in molluscs has led to develop researches for in vitro culture. The chemiluminescence technique has been used to estimate the phagocytosis capacity of Crassostrea gigas hemocytes according to different culture media and times. Sea water was used as a basic medium. Additives, such as antibiotics or glucose and buffers, such as Tris or Hepes, were tested as weIl as artificial sea water. Hemocyte chemiluminescence activities were recorded at t=o in relation to the medium composition. The highest values were observed when hemocytes were kept in sea water without any modification. Whichever medium, chemiluminescence activity was substantially decreased when hemocytes were previously kept in vitro for a few... |
| Tipo: Text |
Palavras-chave: Crassotrea gigas; Oyster; Bonamia ostreae; Pathogens; Molluscs; Hemocytes. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-4115.PDF |
| |
|
| |
|
|
| Boudour-boucheker, Nesrine; Boulo, Viviane; Charmantier-daures, Mireille; Grousset, Evelyse; Anger, Klaus; Charmantier, Guy; Lorin-nebel, Catherine. |
| V-H+-ATPase and Na+/K+-ATPase were localized in the gills and branchiostegites of M. amazonicum and the effects of salinity on the branchial chamber ultrastructure and on the localization of transporters were investigated. Gills present septal and pillar cells. In freshwater (FW), the apical surface of pillar cells is amplified by extensive evaginations associated with mitochondria. V-H+-ATPase immunofluorescence was localized in the membranes of the apical evaginations and in clustered subapical areas of pillar cells, suggesting labeling of intracellular vesicle membranes. Na+/K+-ATPase labeling was restricted to the septal cells. No difference in immunostaining was recorded for both proteins according to salinity (FW vs. 25 PSU). In the branchiostegite,... |
| Tipo: Text |
Palavras-chave: Osmoregulation; Na+/K+-ATPase; V-type H+-ATPase; Gills; Branchiostegite. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00202/31352/37901.pdf |
| |
|
|
| Fleury, Elodie; Moal, Jeanne; Boulo, Viviane; Daniel, Jean-yves; Mazurais, David; Henaut, Alain; Corporeau, Charlotte; Boudry, Pierre; Favrel, Pascal; Huvet, Arnaud. |
| Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. Heredity appears to be a major factor determining the sensitivity of oysters to summer mortality, allowing resistant (R) and susceptible (S) lines to be produced. We conducted genome-wide expression profiling of R and S gonads during the 3-month period preceding a summer mortality event, using a cDNA microarray that we designed. ANOVA analysis revealed that 34 genes were differentially expressed between R and S lines on four dates preceding the mortality event. Annotation of some of these genes highlights reproduction and its allocation and antioxidant defenses as the main pathways that operate differentially... |
| Tipo: Text |
Palavras-chave: Mollusca; Bivalvia; Gene expression; CDNA microarrays; Reproduction; Oxidative stress; Energy metabolism. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00005/11590/8258.pdf |
| |
|
|
| Mialhe, Eric; Boulo, Viviane; Elston, Ralph; Hill, Barry; Hine, Michel; Montes, Jaime; Mont, Jaime; Banning, Paul Van; Grizel, Henri. |
| Murine moncolonal and polyclonal antibodies to antigesn of Bonamia ostreae were assessed for serological comparaison of parasites associated with different geographical originns of flat oyster, Ostrea edulis, and with Tiostrea lutaria. All Bonamia isolates from O. edulis reacted similary with polyclonal and monoclonal antibodies. The parasite associed with Tiostrea lutaria which lacks reactivity with 20 B 2 MAB must be considered as a serologically distinct from of B. ostreae |
| Tipo: Text |
Palavras-chave: Serological comparison; Polyclonal and monoclonal antibodies; Tiostrea lutaria; Ostrea edulis; Bonamia; Comparaison sérologique; Anticorps polyclonaux et monoclonaux; Tiostrea lutaria; Ostrea edulis; Bonamia. |
| Ano: 1988 |
URL: http://archimer.ifremer.fr/doc/1988/publication-3101.pdf |
| |
|
|
| Pham, Dominique; Charmantier, Guy; Wabete, Nelly; Boulo, Viviane; Broutoi, Francis; Mailliez, Jean-rene; Peignon, Jean-marie; Charmantier-daures, Mireille. |
| The ontogeny of osmoregulationwas investigated in Litopenaeus stylirostris by studying salinity tolerance and osmoregulatory capacity. Shrimp at different larval and postlarval stages were exposed to various salinities and survival was monitored for 24 h. Survival rates exceeded 80% at salinity over 25 ppt (750 mOsm.kg(-1)) at all the stages. At salinities below to 25 ppt, salinity tolerance was higher in nauplii and zoeae than in mysis larvae. Postlarval stages were able to withstand lower salinities, e.g. 6.0 ppt (176 mOsm.kg(-1)) at PL9 stage, but they were more sensitive than larvae to salinities over 35 ppt (1035 mOsm.kg(-1)). Zoea and mysis larvae slightly hyper-regulated at all tested salinities. After metamorphosis, postlarvae progressively... |
| Tipo: Text |
Palavras-chave: Penaeid; Litopenaeus stylirostris; Ontogeny; Osmoregulation; Growth. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00107/21843/20058.pdf |
| |
|
|
| Grizel, Henri; Mialhe, Eric; Chagot, Dominique; Boulo, Viviane; Bachere, Evelyne. |
| Research on bonamiasis, an epizootic disease of the edible oyster Ostrea edu/is caused by the protozoan Bonamia ostreae, is discussed in relation to oyster fanning, research technology, epizootiology, and management. Morphological and infectious characteristics of the parasite are described. Recent progress in isolation and purification of the parasite have permitted investigations into host defense mechanisms, parasite infectivity, and the development of a mol1usc-pathogen mode!. |
| Tipo: Text |
Palavras-chave: Epizootic disease; Pathology; Protozoan; Bonamia ostreae; Oysters. |
| Ano: 1988 |
URL: http://archimer.ifremer.fr/doc/1988/publication-3120.pdf |
| |
|
| |
|
|
| Labreuche, Yannick; Chenivesse, Sabine; Jeudy, Alexandra; Le Panse, Sophie; Boulo, Viviane; Ansquer, Dominique; Pages, Sylvie; Givaudan, Alain; Czjzek, Mirjam; Le Roux, Frederique. |
| The Tetraconata (Pancrustacea) concept proposes that insects are more closely related to aquatic crustaceans than to terrestrial centipedes or millipedes. The question therefore arises whether insects have kept crustacean-specific genetic traits that could be targeted by specific toxins. Here we show that a toxin (nigritoxin), originally identified in a bacterial pathogen of shrimp, is lethal for organisms within the Tetraconata and non-toxic to other animals. X-ray crystallography reveals that nigritoxin possesses a new protein fold of the α/β type. The nigritoxin N-terminal domain is essential for cellular translocation and likely encodes specificity for Tetraconata. Once internalized by eukaryotic cells, nigritoxin induces apoptotic cell death through... |
| Tipo: Text |
|
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00408/51934/52563.pdf |
| |
|
| |
|
| |
|
|
| Cochennec, Nathalie; Hervio, Dominique; Panatier, B; Boulo, Viviane; Mialhe, Eric; Rogier, H; Grizel, Henri; Paolucci, F. |
| Currently recognition of molluscan diseases relies heavily upon light microscopic examination of stained histological sections of molluscan tissues Such histopathological methods are time consuming and relatively inefficient In the study of ongoing epizootics Alternatively, more effluent investigative tools are needed to accelerate disease recognition and quantification Such methods are needed to prevent control and eradicate molluscan diseases This paper reports the development of a direct sandwich enzyme-lmmunosorbent assay (ELISA) for the ascetosporan intrahemocytic parasite Bonamiao ostreae of the flat oyster Ostrea edulis. Bonamiasis is a commercially important epizootic disease of oysters A Bonamia-specific monoclonal antibody is employed in the... |
| Tipo: Text |
|
| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/1992/publication-2765.pdf |
| |
|
|
| Alix, Maud; Blondeau-bidet, Eva; Grousset, Evelyse; Shiranghi, Ainaz; Vergnet, Alain; Guinand, Bruno; Chatain, Beatrice; Boulo, Viviane; Lignot, Jehan-herve. |
| Fasting and refeeding occur naturally in predators but this is largely ignored when dealing with farmed fish. Therefore, the effects of 3-week fasting and re-alimentation (2.5% of the individual body mass) were investigated using two genetically selected populations (F2 generation) of 250 g juvenile sea bass (Dicentrarchus labrax L.). Blood osmolarity, gill and intestinal morphology and expression of the sodium pump (Na+, K+-ATPase, NKA) were studied on two phenotypes showing different degrees of body mass loss during food deprivation: one group losing body mass rapidly during fasting (F +) and the other one limiting body mass loss during the same period (F-). Blood osmotic pressure significantly decreases due to re-alimentation in both groups, but this... |
| Tipo: Text |
|
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00354/46521/46285.pdf |
| |
|
|
| Chagot, Dominique; Comps, Michel; Boulo, Viviane; Ruano, Francisco; Grizel, Henri. |
| An infection caused by a Perkinsus -like parasite has been observed in Ruditapes decussatus from Portugal (Comps and Chagot, 1987). Following a pathological study carried out on clams affected by mortalities in the Algarve area, this parasite was found again, inducing a reaction process in the host. Histological examination reveals the parasite in the connective tissue of different organs. The adjoining tissues, particularly the epithelia, are not affected. |
| Tipo: Text |
Palavras-chave: Cells; Histology; Pathology; Ruditapes decussatus. |
| Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/1987/acte-3109.pdf |
| |
|
|
| Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Haure, Joel; Bedier, Edouard; Ropert, Michel; Moal, Jeanne; Huvet, Arnaud; Bacca, Helene; Van Wormhoudt, A; Delaporte, Maryse; Costil, K; Pouvreau, Stephane; Lambert, Christian; Boulo, Viviane; Soudant, Philippe; Nicolas, Jean-louis; Leroux, Frederique; Renault, Tristan; Gagnaire, Beatrice; Geret, Florence; Boutet, Isabelle; Burgeot, Thierry; Boudry, Pierre. |
| Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and biological parameters has been suggested as the cause and is now starting to be identified. A high genetic basis was found for survival in oysters when a first generation (G1) was tested in three sites during summer. This paper presents a synthesis on physiological characteristics of two selected groups ('R' and 'S', from families selected for resistance and susceptibility to summer mortality respectively), of the second and third generations. R and S showed improvement or reduction of survival compared with the control in both field and laboratory trials... |
| Tipo: Text |
Palavras-chave: ROS; Temperature; Stress; Hemocytes; Reproduction; Genetic selection; Resistant oyster; Crassostrea gigas; Pacific Oyster; Summer mortality. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2809.pdf |
| |
|
|
| Boudour-boucheker, Nesrine; Boulo, Viviane; Charmantier-daures, Mireille; Anger, Klaus; Charmantier, Guy; Lorin-nebel, Catherine. |
| In this comparative study, osmoregulatory mechanisms were analyzed in two closely related species of palaemonid shrimp from Brazil, Macrobrachium pantanalense and Macrobrachium amazonicum. A previous investigation showed that all postembryonic stages of M. pantanalense from inland waters of the Pantanal are able to hyper-osmoregulate in fresh water, while this species was not able to hypo-osmoregulate at high salinities. In M. amazonicum originating from the Amazon estuary, in contrast, all stages are able to hypo-osmoregulate, but only first-stage larvae, late juveniles and adults are able to hyper-osmoregulate in fresh water. The underlying molecular mechanisms of these physiological differences have not been known. We therefore investigated the... |
| Tipo: Text |
Palavras-chave: Salinity; Crustaceans; Branchial chamber; NHE3 transporter; V-type H+-ATPase; Na+/K+-ATPase. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00313/42427/41899.pdf |
| |
|
|
| Huvet, Arnaud; Royer, J.; Moal, J.; Burgeot, Thierry; Lapègue, Sylvie; Boulo, Viviane; Nicolas, Jean-louis; Lambert, C.. |
| Les résultats du programme MOREST démontrent l'existence d'une composante génétique à la survie estivale du naissain et nous permettent de disposer aujourd'hui d'huTtres « résistantes» (R) et « susceptibles» (S) aux mortalités estivales (Dégremont et aL, 2002, 2005). La forte héritabilité estimée face à un tel système multifactoriel permet de suspecter que le caractère sélectionné puisse être gouverné par un ou un trés petit nombre de gènes sur une région chromosomique donnée. D'après le modèle d'interaction suspecté entre l'environnement, l'huître et des pathogènes opportunistes, une hiérarchie des facteurs, conduisant aux mortalités, permet de montrer qu'aucun d'entre eux ne peut provoquer à lui seul les mortalités mais que leur combinaison est... |
| Tipo: Text |
|
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/00306/41706/40917.pdf |
| |
| Registros recuperados: 35 | |
|
|
|
