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| Dupont, Samuel; Carre-mlouka, Alyssa; Domart-coulon, Isabelle; Vacelet, Jean; Bourguet-kondracki, Marie-lise. |
| Combining culture-dependent and independent approaches, we investigated for the first time the cultivable fraction of the prokaryotic community associated with the carnivorous sponge Asbestopluma hypogea. The heterotrophic prokaryotes isolated from this tiny sponge were compared between specimens freshly collected from cave and maintained in aquarium. Overall, 67 isolates obtained in pure culture were phylogenetically affiliated to the bacterial phyla Proteobacteria, Bacteroidetes, Actinobacteria, and Firmicutes. This cultivable diversity was lower than the prokaryotic diversity obtained by previous pyrosequencing study and comparable to that of another Mediterranean demosponge, the filter-feeding Phorbas tenacior. Furthermore, using fluorescence in situ... |
| Tipo: Text |
Palavras-chave: Porifera; Asbestopluma hypogea; Carnivorous sponge; Symbiosis; Microbiology; Biologic activities. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00372/48273/48572.pdf |
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| Longeon, Arlette; Copp, Brent R.; Quevrain, Elodie; Roue, Melanie; Kientz, Betty; Cresteil, Thierry; Petek, Sylvain; Debitus, Cecile; Bourguet-kondracki, Marie-lise. |
| Indole derivatives including bromoindoles have been isolated from the South Pacific marine sponges Rhopaloeides odorabile and Hyrtios sp. Their structures were established through analysis of mass spectra and 1D and 2D NMR spectroscopic data. Their potential inhibitory phospholipase A(2) (PLA(2)), antioxidant and cytotoxic activities were evaluated. The new derivative 5,6-dibromo-L-hypaphorine (9) isolated from Hyrtios sp. revealed a weak bee venom PLA2 inhibition (IC50 0.2 mM) and a significant antioxidant activity with an Oxygen Radical Absorbance Capacity (ORAC) value of 0.22. The sesquiterpene aureol (4), also isolated from Hyrtios sp., showed the most potent antioxidant activity with an ORAC value of 0.29. |
| Tipo: Text |
Palavras-chave: Indole derivatives; Bromoindoles; Marine sponge; Rhopaloeides odorabile; Hyrtios sp.; PLA(2) inhibitor; Antioxidant; Cytotoxic. |
| Ano: 2011 |
URL: https://archimer.ifremer.fr/doc/00214/32485/30975.pdf |
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| Dupont, Samuel; Corre, Erwan; Li, Yanyan; Vacelet, Jean; Bourguet-kondracki, Marie-lise. |
| Using 454 pyrosequencing, we characterized for the first time the associated microbial community of the deep-sea carnivorous Demosponge Asbestopluma hypogea (Cladorhizidae). Targeting the 16S rRNA gene V3 and V6 hypervariable regions, we compared the diversity and composition of associated microbes of two individual sponges of A.hypogea freshly collected in the cave with surrounding seawater and with one sponge sample maintained 1year in an aquarium after collection. With more than 22961 high quality sequences from sponge samples, representing c. 800 operational taxonomic units per sponge sample at 97% sequence similarities, the phylogenetic affiliation of A.hypogea-associated microbes was assigned to 20 bacterial and two archaeal phyla, distributed into... |
| Tipo: Text |
Palavras-chave: Asbestopluma hypogea; Carnivorous sponge; Cladorhizidae; Microbial diversity; Microscopic studies; Pyrosequencing 454 amplicons. |
| Ano: 2013 |
URL: https://archimer.ifremer.fr/doc/00372/48277/48534.pdf |
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