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Environmental DNA reveals tropical shark diversity in contrasting levels of anthropogenic impact ArchiMer
Bakker, Judith; Wangensteen, Owen S.; Chapman, Demian D.; Boussarie, Germain; Buddo, Dayne; Guttridge, Tristan L.; Hertler, Heidi; Mouillot, David; Vigliola, Laurent; Mariani, Stefano.
Sharks are charismatic predators that play a key role in most marine food webs. Their demonstrated vulnerability to exploitation has recently turned them into flagship species in ocean conservation. Yet, the assessment and monitoring of the distribution and abundance of such mobile species in marine environments remain challenging, often invasive and resource-intensive. Here we pilot a novel, rapid and non-invasive environmental DNA (eDNA) metabarcoding approach specifically targeted to infer shark presence, diversity and eDNA read abundance in tropical habitats. We identified at least 21 shark species, from both Caribbean and Pacific Coral Sea water samples, whose geographical patterns of diversity and read abundance coincide with geographical differences...
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Ano: 2017 URL: https://archimer.ifremer.fr/doc/00416/52732/74686.pdf
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Seabirds supply nitrogen to reefbuilding corals on remote Pacific islets ArchiMer
Lorrain, Anne; Houlbreque, Fanny; Benzoni, Francesca; Barjon, Lucie; Tremblay-boyer, Laura; Menkes, Christophe; Gillikin, David P.; Payri, Claude; Jourdan, Herve; Boussarie, Germain; Verheyden, Anouk; Vidal, Eric.
Seabirds concentrate nutrients from large marine areas on their nesting islands playing an important ecological role in nutrient transfer between marine and terrestrial ecosystems. Here we investigate the role of guano on corals reefs across scales by analyzing the stable nitrogen isotopic (delta N-15) values of the scleractinian coral Pocillopora damicornis on fringing reefs around two Pacific remote islets with large seabird colonies. Marine stations closest to the seabird colonies had higher nitrate + nitrite concentrations compared to more distant stations. Coral and zooxanthellae delta N-15 values were also higher at these sites, suggesting that guano-derived nitrogen is assimilated into corals and contributes to their nitrogen requirements. The...
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Ano: 2017 URL: https://archimer.ifremer.fr/doc/00391/50239/50878.pdf
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Environmental DNA illuminates the dark diversity of sharks ArchiMer
Boussarie, Germain; Bakker, Judith; Wangensteen, Owen S.; Mariani, Stefano; Bonnin, Lucas; Juhel, Jean-baptiste; Kiszka, Jeremy J.; Kulbicki, Michel; Manel, Stephanie; Robbins, William D.; Vigliola, Laurent; Mouillot, David.
In the era of "Anthropocene defaunation," large species are often no longer detected in habitats where they formerly occurred. However, it is unclear whether this apparent missing, or "dark," diversity of megafauna results from local species extirpations or from failure to detect elusive remaining individuals. We find that despite two orders of magnitude less sampling effort, environmental DNA (eDNA) detects 44% more shark species than traditional underwater visual censuses and baited videos across the New Caledonian archipelago (south-western Pacific). Furthermore, eDNA analysis reveals the presence of previously unobserved shark species in human-impacted areas. Overall, our results highlight a greater prevalence of sharks than described by traditional...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00442/55321/56837.pdf
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Remote reefs and seamounts are the last refuges for marine predators across the Indo-Pacific ArchiMer
Letessier, Tom B.; Mouillot, David; Bouchet, Phil J.; Vigliola, Laurent; Fernandes, Marjorie C.; Thompson, Chris; Boussarie, Germain; Turner, Jemma; Juhel, Jean-baptiste; Maire, Eva; Caley, M. Julian; Koldewey, Heather J.; Friedlander, Alan; Sala, Enric; Meeuwig, Jessica J..
Since the 1950s, industrial fisheries have expanded globally, as fishing vessels are required to travel further afield for fishing opportunities. Technological advancements and fishery subsidies have granted ever-increasing access to populations of sharks, tunas, billfishes, and other predators. Wilderness refuges, defined here as areas beyond the detectable range of human influence, are therefore increasingly rare. In order to achieve marine resources sustainability, large no-take marine protected areas (MPAs) with pelagic components are being implemented. However, such conservation efforts require knowledge of the critical habitats for predators, both across shallow reefs and the deeper ocean. Here, we fill this gap in knowledge across the Indo-Pacific...
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00509/62107/66283.pdf
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