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	Provedor de dados BABT (1) BJID (1) Autor Bouzari,Saeid (2) Oloomi,Mana (2) Aslani,Mohammad M (1) Dashti,Amir (1) Golshani,Maryam (1) Jafari,Anis (1) Mahdian,Reza (1) Salami,Mona (1) Shokrgozar,Mohammad Ali (1) Valiyari,Samira (1) Mais... Palavra-chave Anti-cancer agent (1) Diarrhea (1) Enteropathogenic Escherichia coli (1) Polymerase chain reaction (1) Recombinant production (1) Smallest isoform of IL-24 (1) Thioredoxin (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2017 (1) 2011 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Expression, Purification and Functional Assessment of Smallest Isoform of Human Interleukin-24 in Escherichia coli BABT Valiyari,Samira; Mahdian,Reza; Salami,Mona; Oloomi,Mana; Golshani,Maryam; Shokrgozar,Mohammad Ali; Bouzari,Saeid. ABSTRACT Interleukin-24 (IL-24) is a novel tumor-suppressor gene that has different alternative splice isoforms. It has been shown that new smallest isoform of human IL-24 gene, lacking three exons, induces higher levels of cytotoxicity than all the isoforms, indicating shortest isoform of IL-24 may be a new promising anti-cancer agent. In this study, we aimed to provide a reproducible method for recombinant production of the smallest isoform of IL-24 (sIL-24). The Structure of sIL-24 was analyzed using bioinformatics tools (I-TASSER, Prosa, RAMPAGE and SPDBV version 4.1). The DNA sequence encoding sIL-24 was chemically synthesized and sub-cloned into the pET-32a (+) vector for further protein expression in Escherichia coli BL21 (DE3) strain. Upon IPTG... Tipo: Info:eu-repo/semantics/article Palavras-chave: Smallest isoform of IL-24; Thioredoxin; Recombinant production; Anti-cancer agent. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100307 Comparison of multiplex PCR with serogrouping and PCR-RFLP of fliC gene for the detection of enteropathogenic Escherichia coli (EPEC) BJID Bouzari,Saeid; Aslani,Mohammad M; Oloomi,Mana; Jafari,Anis; Dashti,Amir. Enteropathogenic Escherichia coli (EPEC) comprise one of the six categories of diarrhoeagenic E. coli (DEC). EPEC is subgrouped into typical (tEPEC) and atypical (aEPEC). The identification of DEC cannot be based only on cultural and biochemical criteria, since they are indistinguishable from the non-pathogenic E. coli commonly found in human feces. Several PCR methods, with both single and multiple target genes, have been reported for detecting the different DEC pathotypes. In the present study five hundred E. coli isolates from children with diarrhea were subjected into multiplex PCR. Furthermore the strains were typed serologically with O antisera and their fliC gene was characterized by PCR-RFLP. The results obtained revealed that overall 41 (8.2%)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Diarrhea; Enteropathogenic Escherichia coli; Polymerase chain reaction. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400011 Registros recuperados: 2 Primeira ... 1 ... Última

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