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| Brandão,Paulo E.; Hora,Aline S.; Silva,Sheila O. S.; Taniwaki,Sueli A.; Berg,Mikael. |
| Abstract Avian coronavirus (AvCoV) is ubiquitously present on poultry as a multitude of virus lineages. Studies on AvCoV phenotypic traits are dependent on the isolation of field strains in chicken embryonated eggs, but the mutant spectrum on each isolate is not considered. This manuscript reports the previously unknown HTS (high throughput sequencing)-based complete genome haplotyping of AvCoV isolates after passages of two field strains in chicken embryonated eggs. For the first and third passages of strain 23/2013, virus loads were 6.699 log copies/ μL and 6 log copies/ μL and, for 38/2013, 5.699 log copies/μL and 2.699 log copies/μL of reaction, respectively. The first passage of strain 23/2013 contained no variant haplotype, while, for the third... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avian coronavirus; Haplotype; Mutant spectrum; NGS; Evolution. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400202 |
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| Mori,Enio; Lara,Maria do Carmo C.S.H.; Cunha,Elenice M.S.; Villalobos,Eliana M.C.; Mori,Claudia M.C.; Soares,Rodrigo M.; Brandão,Paulo E.; Fernandes,Wilson R.; Richtzenhain,Leonardo J.. |
| Partial nucleotide sequences of ORF72 (glycoprotein D, gD), ORF64 (infected cell protein 4, ICP4) and ORF30 (DNA polymerase) genes were compared with corresponding sequences of EHV-1 reference strains to characterize the molecular variability of Brazilian strains. Virus isolation assays were applied to 74 samples including visceral tissue, total blood, cerebrospinal fluid (CSF) and nasal swabs of specimens from a total of 64 animals. Only one CSF sample (Iso07/05 strain) was positive by virus isolation in cell culture. EHV-1 Iso07/05 neurologic strain and two abortion visceral tissues samples (Iso11/06 and Iso33/06) were PCR-positive for ORF33 (glycoprotein B, gB) gene of EHV-1. A sequence analysis of the ORF72, ORF64 and ORF30 genes from three EHV-1... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Equid herpesvirus type 1; Equid Brazilian herpesvirus; ICP4 gene (ORF64); Glycoprotein D gene (ORF72); DNA polymerase gene (ORF30). |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200565 |
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| Ono,Ekaterina A. Durymanova; Iamamoto,Keila; Castilho,Juliana G.; Carnieli Jr.,Pedro; Oliveira,Rafael de Novaes; Achkar,Samira M.; Carrieri,Maria L.; Kotait,Ivanete; Brandão,Paulo E.. |
| Rabies is a zoonotic disease that affects all mammals and leads to more than 55,000 human deaths every year, caused by rabies virus (RABV) (Mononegavirales: Rhabdoviridae: Lyssavirus). Currently, human rabies treatment is based on the Milwaukee Protocol which consists on the induction of coma and massive antiviral therapy. The aim of this study was to assess the decrease in the titer of rabies virus both in vitro and in vivo using short-interfering RNAs. To this end, three siRNAs were used with antisense strands complementary to rabies virus nucleoprotein (N) mRNA. BHK-21 cells monolayers were infected with 1000 to 0.1 TCID50 of PV and after 2 hours the cells were transfected with each of tree RNAs in separate using Lipofectamine-2000. All three siRNAs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rabies; RNA interference; SiRNAs; Antiviral; Treatment. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300034 |
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